| pubmed-article:15851482 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15851482 | lifeskim:mentions | umls-concept:C0596995 | lld:lifeskim |
| pubmed-article:15851482 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:15851482 | lifeskim:mentions | umls-concept:C0038952 | lld:lifeskim |
| pubmed-article:15851482 | lifeskim:mentions | umls-concept:C1414205 | lld:lifeskim |
| pubmed-article:15851482 | lifeskim:mentions | umls-concept:C0086597 | lld:lifeskim |
| pubmed-article:15851482 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:15851482 | pubmed:issue | 27 | lld:pubmed |
| pubmed-article:15851482 | pubmed:dateCreated | 2005-7-4 | lld:pubmed |
| pubmed-article:15851482 | pubmed:abstractText | The kinase Mirk/dyrk1B is essential for the differentiation of C2C12 myoblasts. Mirk reinforces the G0/G1 arrest state in which differentiation occurs by directly phosphorylating and stabilizing p27(Kip1) and destabilizing cyclin D1. We now demonstrate that Mirk is anti-apoptotic in myoblasts. Knockdown of endogenous Mirk by RNA interference activated caspase 3 and decreased myoblast survival by 75%, whereas transient overexpression of Mirk increased cell survival. Mirk exerts its anti-apoptotic effects during muscle differentiation at least in part through effects on the cell cycle inhibitor and pro-survival molecule p21(Cip1). Overexpression and RNA interference experiments demonstrated that Mirk phosphorylates p21 within its nuclear localization domain at Ser-153 causing a portion of the typically nuclear p21 to localize in the cytoplasm. Phosphomimetic GFP-p21-S153D was pancellular in both cycling C2C12 myoblasts and NIH3T3 cells. Endogenous Mirk in myotubes and overexpressed Mirk in NIH3T3 cells were able to cause the pancellular localization of wild-type GFP-p21 but not the nonphosphorylatable mutant GFP-p21-S153A. Translocation to the cytoplasm enables p21 to block apoptosis through inhibitory interaction with pro-apoptotic molecules. Phosphomimetic p21-S153D was more effective than wild-type p21 in blocking the activation of caspase 3. Transient expression of p21-S153D also increased myoblast viability in colony forming assays, whereas the p21-S153A mutant had no effect. This Mirk-dependent change in p21 intracellular localization is a natural part of myoblast differentiation. Endogenous p21 localized exclusively to the nuclei of proliferating myoblasts but was also found in the cytoplasm of post-mitotic multinucleated myotubes and adult human skeletal myofibers. | lld:pubmed |
| pubmed-article:15851482 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15851482 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15851482 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15851482 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:15851482 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:15851482 | pubmed:author | pubmed-author:DengXiaobingX | lld:pubmed |
| pubmed-article:15851482 | pubmed:author | pubmed-author:FriedmanEilee... | lld:pubmed |
| pubmed-article:15851482 | pubmed:author | pubmed-author:EwtonDaina... | lld:pubmed |
| pubmed-article:15851482 | pubmed:author | pubmed-author:LimSeunghwanS | lld:pubmed |
| pubmed-article:15851482 | pubmed:author | pubmed-author:MercerStephen... | lld:pubmed |
| pubmed-article:15851482 | pubmed:author | pubmed-author:MazurThomas... | lld:pubmed |
| pubmed-article:15851482 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15851482 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:15851482 | pubmed:volume | 280 | lld:pubmed |
| pubmed-article:15851482 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15851482 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15851482 | pubmed:pagination | 25788-801 | lld:pubmed |
| pubmed-article:15851482 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:15851482 | pubmed:meshHeading | pubmed-meshheading:15851482... | lld:pubmed |
| pubmed-article:15851482 | pubmed:meshHeading | pubmed-meshheading:15851482... | lld:pubmed |
| pubmed-article:15851482 | pubmed:meshHeading | pubmed-meshheading:15851482... | lld:pubmed |
