| pubmed-article:15824058 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15824058 | lifeskim:mentions | umls-concept:C0335038 | lld:lifeskim |
| pubmed-article:15824058 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
| pubmed-article:15824058 | lifeskim:mentions | umls-concept:C0872079 | lld:lifeskim |
| pubmed-article:15824058 | lifeskim:mentions | umls-concept:C0013682 | lld:lifeskim |
| pubmed-article:15824058 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:15824058 | pubmed:dateCreated | 2005-4-12 | lld:pubmed |
| pubmed-article:15824058 | pubmed:abstractText | Methods for determining protein-protein interactions in mammalian cells typically rely on single reporter functions and are susceptible to variations between samples particularly in regard to levels of transcription, processing and translation. A method has been developed for determining protein-protein interactions in mammalian cells, which bypasses these variables confounding single reporter assays. The approach utilizes two units of gene expression linked to reporter functions that are interposed by a deactivation-activation unit in such a way that the downstream expression unit is switched off. Hence upstream expression occurs regardless of protein-protein interaction, leading to the production of the upstream reporter. In the event of protein-protein interactions, the downstream expression unit is switched on leading to dual reporter read outs. Thus, the ratio of the two reporter activities provides a measure to determine the efficiency of protein-protein interactions. To access the system we screened a mutant of BMPR2 where the interaction between BMPR-II and LIMK is abrogated. BMPR-II is a type II receptor of the TGFbeta superfamily and plays a key role in the pathogenesis of familial pulmonary arterial hypertension. This system has potential for high-throughput screening of libraries (peptide, chemical, cDNA, etc.) to isolate agents that are capable of interfering with highly selective protein-protein interaction. | lld:pubmed |
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| pubmed-article:15824058 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15824058 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15824058 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15824058 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15824058 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15824058 | pubmed:issn | 1362-4962 | lld:pubmed |
| pubmed-article:15824058 | pubmed:author | pubmed-author:TrembathR CRC | lld:pubmed |
| pubmed-article:15824058 | pubmed:author | pubmed-author:NasimM TMT | lld:pubmed |
| pubmed-article:15824058 | pubmed:issnType | Electronic | lld:pubmed |
| pubmed-article:15824058 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:15824058 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15824058 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15824058 | pubmed:pagination | e66 | lld:pubmed |
| pubmed-article:15824058 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:meshHeading | pubmed-meshheading:15824058... | lld:pubmed |
| pubmed-article:15824058 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15824058 | pubmed:articleTitle | A dual-light reporter system to determine the efficiency of protein-protein interactions in mammalian cells. | lld:pubmed |
| pubmed-article:15824058 | pubmed:affiliation | Department of Genetics, University of Leicester, Leicester LE1 7RH, UK. mtn4@le.ac.uk | lld:pubmed |
| pubmed-article:15824058 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15824058 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15824058 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| entrez-gene:659 | entrezgene:pubmed | pubmed-article:15824058 | lld:entrezgene |
