| pubmed-article:15745752 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C0003374 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C0021467 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C0220908 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C1552603 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C1706202 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C0062496 | lld:lifeskim |
| pubmed-article:15745752 | lifeskim:mentions | umls-concept:C0205198 | lld:lifeskim |
| pubmed-article:15745752 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:15745752 | pubmed:dateCreated | 2005-3-4 | lld:pubmed |
| pubmed-article:15745752 | pubmed:abstractText | Antimalarial drugs such as chloroquine are believed to act by inhibiting hemozoin formation in the food vacuole of the malaria parasite. We have developed a new assay for measuring and detecting inhibition of synthetic hemozoin (beta-hematin) formation. Aqueous pyridine (5% v/v, pH 7.5) forms a low-spin complex with hematin but not with beta-hematin. Its absorbance obeys Beer's law, making it useful for quantitating hematin concentration in hematin/beta-hematin mixtures, allowing compounds to be investigated for inhibition of beta-hematin formation. The assay is rapid (60 min incubation) and requires no centrifugation. The beta-hematin inhibition data show good agreement with alternative assay methods reported by four laboratories. The assay was adapted for high-throughput colorimetric screening, allowing visual identification of beta-hematin inhibitors. In this mode, the assay successfully detected all 18 beta-hematin inhibitors in a set of 47 compounds tested, with no false positive results. The quantitative in vitro antimalarial activities of a set of 13 aminoquinolines and quinoline methanols were found to correlate significantly with beta-hematin inhibition values determined using the assay. | lld:pubmed |
| pubmed-article:15745752 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15745752 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15745752 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15745752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15745752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15745752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15745752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15745752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15745752 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15745752 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15745752 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15745752 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:15745752 | pubmed:author | pubmed-author:EganTimothy... | lld:pubmed |
| pubmed-article:15745752 | pubmed:author | pubmed-author:NcokaziKanyil... | lld:pubmed |
| pubmed-article:15745752 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15745752 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:15745752 | pubmed:volume | 338 | lld:pubmed |
| pubmed-article:15745752 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15745752 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15745752 | pubmed:pagination | 306-19 | lld:pubmed |
| pubmed-article:15745752 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:meshHeading | pubmed-meshheading:15745752... | lld:pubmed |
| pubmed-article:15745752 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15745752 | pubmed:articleTitle | A colorimetric high-throughput beta-hematin inhibition screening assay for use in the search for antimalarial compounds. | lld:pubmed |
| pubmed-article:15745752 | pubmed:affiliation | Department of Chemistry, University of Cape Town, Private Bag, Rondebosch 7701, South Africa. | lld:pubmed |
| pubmed-article:15745752 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15745752 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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