| pubmed-article:15741345 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15741345 | lifeskim:mentions | umls-concept:C0025252 | lld:lifeskim |
| pubmed-article:15741345 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15741345 | lifeskim:mentions | umls-concept:C1332397 | lld:lifeskim |
| pubmed-article:15741345 | lifeskim:mentions | umls-concept:C0162768 | lld:lifeskim |
| pubmed-article:15741345 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:15741345 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:15741345 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15741345 | pubmed:dateCreated | 2005-3-17 | lld:pubmed |
| pubmed-article:15741345 | pubmed:abstractText | An Escherichia coli plasmid vector for the high-level expression of hydrophobic membrane proteins is described. The plasmid, pBCL, directs the expression of a target polypeptide fused to the C terminus of a mutant form of the anti-apoptotic Bcl-2 family protein, Bcl-XL, where the hydrophobic C terminus has been deleted, and Met residues have been mutated to Leu to facilitate CNBr cleavage after a single Met inserted at the beginning of the target sequence. Fusion protein expression is in inclusion bodies, simplifying the protein purification steps. Here we report the high-level production of PLM, a membrane protein that is a member of the FXYD family of tissue-specific and physiological-state-specific auxiliary subunits of the Na,K-ATPase, expressed abundantly in heart and skeletal muscle. We demonstrate that milligram quantities of pure, isotopically labeled protein can be obtained easily and in little time with this system. | lld:pubmed |
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| pubmed-article:15741345 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15741345 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15741345 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15741345 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15741345 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15741345 | pubmed:issn | 0961-8368 | lld:pubmed |
| pubmed-article:15741345 | pubmed:author | pubmed-author:MarassiFrance... | lld:pubmed |
| pubmed-article:15741345 | pubmed:author | pubmed-author:FranzinCarla... | lld:pubmed |
| pubmed-article:15741345 | pubmed:author | pubmed-author:ChoiJungyuenJ | lld:pubmed |
| pubmed-article:15741345 | pubmed:author | pubmed-author:ThaiKhangK | lld:pubmed |
| pubmed-article:15741345 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15741345 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:15741345 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15741345 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15741345 | pubmed:pagination | 948-55 | lld:pubmed |
| pubmed-article:15741345 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:meshHeading | pubmed-meshheading:15741345... | lld:pubmed |
| pubmed-article:15741345 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15741345 | pubmed:articleTitle | Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins. | lld:pubmed |
| pubmed-article:15741345 | pubmed:affiliation | The Burnham Institute, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA. | lld:pubmed |
| pubmed-article:15741345 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15741345 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15741345 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:15741345 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15741345 | pubmed:publicationType | Research Support, N.I.H., Extramural | lld:pubmed |
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