| pubmed-article:1573850 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C1521816 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0227651 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0311400 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C1521970 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:1573850 | lifeskim:mentions | umls-concept:C0549178 | lld:lifeskim |
| pubmed-article:1573850 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:1573850 | pubmed:dateCreated | 1992-6-4 | lld:pubmed |
| pubmed-article:1573850 | pubmed:abstractText | Mesangial cells serve many functions in the glomerulus, including regulation of glomerular ultrafiltration coefficient, matrix production, and eicosanoid generation. The glomerulus is a vascular bed, and the mesangial cell is continually exposed to rhythmic alterations in intraglomerular pressure. Since increased intraglomerular pressure has been implicated as a potential causative agent in the ultimate development of nephrosclerosis, we sought to determine the effect of continuous stretch-relaxation upon parameters of mesangial cell growth and function. Early passage (2-4) cultured rat mesangial cells were plated onto either rigid-bottom or flexible-bottom culture plates coated with type I collagen. After cell attachment, the cells on flexible supports were exposed to continuous stretch-relaxation for 72 to 96 hours at a rate of 100 cycles/minutes at an applied pressure of 7 to 8 KPa (53 to 61 mm Hg). Cellular morphology was altered by continuous stretch-relaxation, with the majority of mesangial cells presenting stellate or straplike morphology. Fluorescein isothiocyanate-labeled phalloidin staining indicated an increase in density of actin filaments running the long axis of the cell. Stretch-relaxation resulted in an approximately 50% increase in cell number. Prostaglandin production, assessed as irPGE2 production, was increased by stretching in mesangial cells from 28 +/- 1 to 49 +/- 4 pg/10(6) cells (N = 12; p less than 0.005). Mechanical stretch/relaxation increased the percentage of protein representing collagenous proteins from 47 +/- 6% to 70 +/- 4%, as assessed by collagenase susceptibility (p less than 0.025). Analysis of pepsin-resistant proteins synthesized indicated that stretch/relaxation resulted in increases in the relative amounts of types I and III collagens produced/cell. Additionally, stretch/relaxation selectively increased the relative amount of type I-homotrimers produced. Thus, when mesangial cells are exposed to cyclic stretch/relaxation, they exhibit significant alterations in morphology, growth, prostaglandin and collagen production. | lld:pubmed |
| pubmed-article:1573850 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1573850 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1573850 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1573850 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1573850 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1573850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1573850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1573850 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1573850 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1573850 | pubmed:month | May | lld:pubmed |
| pubmed-article:1573850 | pubmed:issn | 0023-6837 | lld:pubmed |
| pubmed-article:1573850 | pubmed:author | pubmed-author:HarrisR CRC | lld:pubmed |
| pubmed-article:1573850 | pubmed:author | pubmed-author:HaralsonM AMA | lld:pubmed |
| pubmed-article:1573850 | pubmed:author | pubmed-author:BadrK FKF | lld:pubmed |
| pubmed-article:1573850 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1573850 | pubmed:volume | 66 | lld:pubmed |
| pubmed-article:1573850 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1573850 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1573850 | pubmed:pagination | 548-54 | lld:pubmed |
| pubmed-article:1573850 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:meshHeading | pubmed-meshheading:1573850-... | lld:pubmed |
| pubmed-article:1573850 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1573850 | pubmed:articleTitle | Continuous stretch-relaxation in culture alters rat mesangial cell morphology, growth characteristics, and metabolic activity. | lld:pubmed |
| pubmed-article:1573850 | pubmed:affiliation | Department of Medicine, Vanderbilt University School of Medicine, Nashville, Tennessee. | lld:pubmed |
| pubmed-article:1573850 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1573850 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1573850 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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