| pubmed-article:15725035 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15725035 | lifeskim:mentions | umls-concept:C1511726 | lld:lifeskim |
| pubmed-article:15725035 | lifeskim:mentions | umls-concept:C0037812 | lld:lifeskim |
| pubmed-article:15725035 | lifeskim:mentions | umls-concept:C0598986 | lld:lifeskim |
| pubmed-article:15725035 | lifeskim:mentions | umls-concept:C1706701 | lld:lifeskim |
| pubmed-article:15725035 | lifeskim:mentions | umls-concept:C2347299 | lld:lifeskim |
| pubmed-article:15725035 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:15725035 | pubmed:dateCreated | 2005-2-23 | lld:pubmed |
| pubmed-article:15725035 | pubmed:abstractText | An approach for recording four-dimensional (4D) methyl (1)H-(13)C-(13)C-(1)H NOESY spectra with high resolution and sensitivity is presented and applied to Malate Synthase G (723 residues, 82 kDa). Sensitivity and resolution have been optimized using a highly deuterated, methyl-protonated sample in concert with methyl-TROSY, sparse data sampling in the three indirect dimensions, and 4D spectral reconstruction using multidimensional decomposition (MDD). A sparse data acquisition protocol is introduced that ensures that sufficiently long indirect acquisition times can be employed to exploit the decreased relaxation rates associated with methyl-TROSY, without increasing the duration of the 4D experiment beyond acceptable measurement times. In this manner, only a fraction ( approximately 30%) of the experimental data that would normally be needed to achieve a spectrum of high resolution is acquired. The reconstructed 4D spectrum is of similar resolution and sensitivity to three-dimensional (3D) (13)C-edited NOE spectra, is straightforward to analyze, and resolves ambiguities that emerge when 3D data sets only are considered. | lld:pubmed |
| pubmed-article:15725035 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15725035 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15725035 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15725035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15725035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15725035 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15725035 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15725035 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15725035 | pubmed:issn | 0002-7863 | lld:pubmed |
| pubmed-article:15725035 | pubmed:author | pubmed-author:TugarinovVita... | lld:pubmed |
| pubmed-article:15725035 | pubmed:author | pubmed-author:KayLewis ELE | lld:pubmed |
| pubmed-article:15725035 | pubmed:author | pubmed-author:OrekhovVladis... | lld:pubmed |
| pubmed-article:15725035 | pubmed:author | pubmed-author:IbraghimovIlg... | lld:pubmed |
| pubmed-article:15725035 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15725035 | pubmed:day | 2 | lld:pubmed |
| pubmed-article:15725035 | pubmed:volume | 127 | lld:pubmed |
| pubmed-article:15725035 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15725035 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15725035 | pubmed:pagination | 2767-75 | lld:pubmed |
| pubmed-article:15725035 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15725035 | pubmed:meshHeading | pubmed-meshheading:15725035... | lld:pubmed |
| pubmed-article:15725035 | pubmed:meshHeading | pubmed-meshheading:15725035... | lld:pubmed |
| pubmed-article:15725035 | pubmed:meshHeading | pubmed-meshheading:15725035... | lld:pubmed |
| pubmed-article:15725035 | pubmed:meshHeading | pubmed-meshheading:15725035... | lld:pubmed |
| pubmed-article:15725035 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15725035 | pubmed:articleTitle | High-resolution four-dimensional 1H-13C NOE spectroscopy using methyl-TROSY, sparse data acquisition, and multidimensional decomposition. | lld:pubmed |
| pubmed-article:15725035 | pubmed:affiliation | Protein Engineering Network Centers of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, The University of Toronto, Toronto, Ontario M5S 1A8, Canada. | lld:pubmed |
| pubmed-article:15725035 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15725035 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15725035 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15725035 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15725035 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15725035 | lld:pubmed |
