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pubmed-article:15698607pubmed:dateCreated2005-2-8lld:pubmed
pubmed-article:15698607pubmed:abstractTextIsovaleric acidemia (IVA) is one of the various target disorders for tandem mass spectrometry (MS/MS) newborn screening. In the diagnosis of IVA, no enzymatic assay method for isovaleryl-CoA dehydrogenase (IVD) activity has been reported whereby the production of enoyl-CoA species was directly detected. We established a direct assay method to detect 3-methylcrotonyl-CoA (MC-CoA) production using high-performance liquid chromatography (HPLC).lld:pubmed
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pubmed-article:15698607pubmed:issn0009-8981lld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:KobayashiMasa...lld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:ShigematsuYos...lld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:OnoHiroakiHlld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:SakamotoAkiko...lld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:SakuraNobuoNlld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:NishimuraYuta...lld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:TajimaGoGlld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:YofuneHirokoHlld:pubmed
pubmed-article:15698607pubmed:authorpubmed-author:Dwi Bahagia...lld:pubmed
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pubmed-article:15698607pubmed:volume353lld:pubmed
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pubmed-article:15698607pubmed:pagination193-9lld:pubmed
pubmed-article:15698607pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:15698607pubmed:year2005lld:pubmed
pubmed-article:15698607pubmed:articleTitleEstablishment of a practical enzymatic assay method for determination of isovaleryl-CoA dehydrogenase activity using high-performance liquid chromatography.lld:pubmed
pubmed-article:15698607pubmed:affiliationDepartment of Pediatrics, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan. ugoch@mac.comlld:pubmed
pubmed-article:15698607pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15698607pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed