| pubmed-article:15684663 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15684663 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:15684663 | lifeskim:mentions | umls-concept:C0014441 | lld:lifeskim |
| pubmed-article:15684663 | lifeskim:mentions | umls-concept:C2702333 | lld:lifeskim |
| pubmed-article:15684663 | lifeskim:mentions | umls-concept:C0301869 | lld:lifeskim |
| pubmed-article:15684663 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:15684663 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:15684663 | pubmed:dateCreated | 2005-2-1 | lld:pubmed |
| pubmed-article:15684663 | pubmed:abstractText | Enzyme-antibody (Ab) conjugates specific for IgG are widely used in indirect immunological assays and have been until recently routinely prepared with polyclonal IgG-specific animal Abs. The use of monoclonal Abs (MAbs) could permit a better standardization of the IgG-specific conjugate reagents but is expected to result in lower reactivity due to the recognition of a single epitope by the MAbs. In this work, we have characterized a monoclonal anti-human IgG-peroxidase (HRP) reagent and compared its reactivity with commercial reagents. The murine C5-1 anti-human IgG MAb was selected for conjugation because of its high affinity (K(a) = 1.9 x 10(10)M), pan-IgG reactivity and absence of cross-reactivity with various structures including animal IgGs. The specific activity and binding kinetics of the C5-1:HRP conjugate were similar to the ones of two polyclonal anti-IgG:HRP conjugates when tested with immobilized human IgG. The C5-1:HRP conjugate could detect low amounts of human IgG much more effectively than two commercial monoclonal conjugates although it was slightly less effective than a polyclonal conjugate. However, the C5-1 conjugate yielded reduced background reactivity compared to the polyclonal conjugate, resulting in similar signal-to-noise ratios. These results indicate that the C5-1:HRP conjugate could be a suitable substitute for anti-human IgG conjugates prepared from animal antisera. | lld:pubmed |
| pubmed-article:15684663 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15684663 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15684663 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15684663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15684663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15684663 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15684663 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15684663 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:15684663 | pubmed:issn | 1536-8599 | lld:pubmed |
| pubmed-article:15684663 | pubmed:author | pubmed-author:LemieuxRéalR | lld:pubmed |
| pubmed-article:15684663 | pubmed:author | pubmed-author:ChevrierMarie... | lld:pubmed |
| pubmed-article:15684663 | pubmed:author | pubmed-author:ChâteauneufIs... | lld:pubmed |
| pubmed-article:15684663 | pubmed:author | pubmed-author:GuérinMatthie... | lld:pubmed |
| pubmed-article:15684663 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15684663 | pubmed:volume | 23 | lld:pubmed |
| pubmed-article:15684663 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15684663 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15684663 | pubmed:pagination | 362-7 | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:meshHeading | pubmed-meshheading:15684663... | lld:pubmed |
| pubmed-article:15684663 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15684663 | pubmed:articleTitle | Sensitive detection of human IgG in ELISA using a monoclonal anti-IgG-peroxidase conjugate. | lld:pubmed |
| pubmed-article:15684663 | pubmed:affiliation | Research and Development, HEMA-QUEBEC, Québec, Canada. | lld:pubmed |
| pubmed-article:15684663 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15684663 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15684663 | lld:pubmed |
