| pubmed-article:15680153 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15680153 | lifeskim:mentions | umls-concept:C0004358 | lld:lifeskim |
| pubmed-article:15680153 | lifeskim:mentions | umls-concept:C0439605 | lld:lifeskim |
| pubmed-article:15680153 | lifeskim:mentions | umls-concept:C1707391 | lld:lifeskim |
| pubmed-article:15680153 | lifeskim:mentions | umls-concept:C0751719 | lld:lifeskim |
| pubmed-article:15680153 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:15680153 | pubmed:dateCreated | 2005-1-31 | lld:pubmed |
| pubmed-article:15680153 | pubmed:abstractText | Autoantibodies against chromatin are the most characteristic serological feature in SLE patients. Anti-dsDNA and nucleosome-specific antibodies are associated with glomerulonephritis, the most serious manifestation of SLE. Identification of peptides mimicking conformational epitopes (so-called mimotopes) on the nucleosome recognized by these antibodies is of considerable interest. Using an approach similar to that used previously to characterize mimotopes for anti-DNA autoantibodies, we have selected and identified a mimotope for a nucleosome-specific autoantibody (#32) by screening a random peptide phage display library. However, the reactivity of monoclonal antibody (mAb) #32 with the selected mimotope (MIMO#0) in ELISA was dependent on the blocking reagents used. Using nonfat dry milk (5%), mAb #32 clearly bound to MIMO#0, but using fetal bovine calf serum (FCS) (5%), there was no binding. Furthermore, again dependent on the blocking reagent used in ELISA, the selected mimotope MIMO#0 was not only recognized by the selecting antibody mAb #32, but also by a large number of other monoclonal anti-DNA, anti-histone and nucleosome-specific autoantibodies (NSA). We could demonstrate that the selected mimotope was able to bind directly to nucleosomal material (DNA/histone complexes) and labeled DNA. This finding was extended to other previously identified mimotopes for anti-DNA autoantibodies. We conclude that nucleosomal material (DNA/histone complexes), derived from reagents used during the mimotope selection procedure, resulted in the selection of DNA-binding peptides from the phage display library, rather than mimotopes. In addition, we could demonstrate that blocking reagents greatly influence the reactivity of anti-DNA, anti-histone and nucleosome-specific autoantibodies in ELISA. Development of blocking reagents devoid of nucleosomal material (DNA/histone complexes) is urgently needed for assay systems in which anti-nuclear autoantibodies are tested. | lld:pubmed |
| pubmed-article:15680153 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15680153 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15680153 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15680153 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15680153 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15680153 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15680153 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15680153 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15680153 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15680153 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15680153 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:15680153 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:MullerSylvian... | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:PuttermanChai... | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:BerdenJo HJH | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:van der... | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:MonestierMarc... | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:SunYong-Jiang... | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:JacobsCor WCW | lld:pubmed |
| pubmed-article:15680153 | pubmed:author | pubmed-author:DiekerJürgen... | lld:pubmed |
| pubmed-article:15680153 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15680153 | pubmed:volume | 296 | lld:pubmed |
| pubmed-article:15680153 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15680153 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15680153 | pubmed:pagination | 83-93 | lld:pubmed |
| pubmed-article:15680153 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
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| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:meshHeading | pubmed-meshheading:15680153... | lld:pubmed |
| pubmed-article:15680153 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15680153 | pubmed:articleTitle | Mimotopes for lupus-derived anti-DNA and nucleosome-specific autoantibodies selected from random peptide phage display libraries: facts and follies. | lld:pubmed |
| pubmed-article:15680153 | pubmed:affiliation | Nephrology Research Laboratory, Nijmegen Center for Molecular Life Sciences and Division of Nephrology (545), University Medical Center, 6500 HB Nijmegen, The Netherlands. | lld:pubmed |
| pubmed-article:15680153 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15680153 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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