| pubmed-article:15670774 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15670774 | lifeskim:mentions | umls-concept:C0105770 | lld:lifeskim |
| pubmed-article:15670774 | lifeskim:mentions | umls-concept:C0007102 | lld:lifeskim |
| pubmed-article:15670774 | lifeskim:mentions | umls-concept:C0034392 | lld:lifeskim |
| pubmed-article:15670774 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:15670774 | lifeskim:mentions | umls-concept:C0037083 | lld:lifeskim |
| pubmed-article:15670774 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:15670774 | lifeskim:mentions | umls-concept:C1999216 | lld:lifeskim |
| pubmed-article:15670774 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15670774 | pubmed:dateCreated | 2005-1-26 | lld:pubmed |
| pubmed-article:15670774 | pubmed:abstractText | Dysregulation of Wnt/beta-catenin pathway plays a central role in early events in colorectal carcinogenesis. We examined the effect of quercetin, a famous anti-tumor agent, against beta-catenin/Tcf signaling in SW480 cells. Quercetin inhibited the transcriptional activity of beta-catenin/Tcf in SW480 and also in HEK293 cells transiently transfected with constitutively active mutant beta-catenin gene, whose product is not induced to be degraded by APC-Axin-GSK3beta complex, so we concluded that its inhibitory mechanism was related to beta-catenin itself or downstream components. To investigate the precise inhibitory mechanism, we performed EMSA showing that binding of the Tcf complexes to its specific DNA-binding sites was strongly suppressed by quercetin. Immunoprecipitation analysis also showed that the binding of beta-catenin to Tcf-4 was also disrupted by quercetin. Western blot analysis proved these decreased bindings resulted from decreased level of beta-catenin and Tcf-4 product in nucleus caused by quercetin. Together, we suggest that quercetin is an excellent inhibitor of beta-catenin/Tcf signaling in SW480 cell lines, and the reduced beta-catenin/Tcf transcriptional activity is due to the decreased nuclear beta-catenin and Tcf-4 proteins. | lld:pubmed |
| pubmed-article:15670774 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15670774 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15670774 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15670774 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15670774 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15670774 | pubmed:issn | 0006-291X | lld:pubmed |
| pubmed-article:15670774 | pubmed:author | pubmed-author:KimHyun-Kyung... | lld:pubmed |
| pubmed-article:15670774 | pubmed:author | pubmed-author:ParkSeyeonS | lld:pubmed |
| pubmed-article:15670774 | pubmed:author | pubmed-author:YangChul... | lld:pubmed |
| pubmed-article:15670774 | pubmed:author | pubmed-author:ParkChi... | lld:pubmed |
| pubmed-article:15670774 | pubmed:author | pubmed-author:ChangJoon... | lld:pubmed |
| pubmed-article:15670774 | pubmed:author | pubmed-author:HahmEun... | lld:pubmed |
| pubmed-article:15670774 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15670774 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:15670774 | pubmed:volume | 328 | lld:pubmed |
| pubmed-article:15670774 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15670774 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15670774 | pubmed:pagination | 227-34 | lld:pubmed |
| pubmed-article:15670774 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:15670774 | pubmed:meshHeading | pubmed-meshheading:15670774... | lld:pubmed |
| pubmed-article:15670774 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15670774 | pubmed:articleTitle | Quercetin, a potent inhibitor against beta-catenin/Tcf signaling in SW480 colon cancer cells. | lld:pubmed |
| pubmed-article:15670774 | pubmed:affiliation | Division of Chemistry and Molecular Engineering, Seoul National University, Seoul 151-742, Republic of Korea. | lld:pubmed |
| pubmed-article:15670774 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15670774 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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