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pubmed-article:15639245pubmed:abstractTextAlternansucrase (EC 2.4.1.140) is a d-glucansucrase that synthesizes an alternating alpha-(1-->3), (1-->6)-linked d-glucan from sucrose. It also synthesizes oligosaccharides via d-glucopyranosyl transfer to various acceptor sugars. Two of the more efficient monosaccharide acceptors are D-tagatose and L-glucose. In the presence of d-tagatose, alternansucrase produced the disaccharide alpha-d-glucopyranosyl-(1-->1)-beta-D-tagatopyranose via glucosyl transfer. This disaccharide is analogous to trehalulose. We were unable to isolate a disaccharide product from L-glucose, but the trisaccharide alpha-D-glucopyranosyl-(1-->6)-alpha-d-glucopyranosyl-(1-->4)-l-glucose was isolated and identified. This is analogous to panose, one of the structural units of pullulan, in which the reducing-end D-glucose residue has been replaced by its L-enantiomer. The putative L-glucose disaccharide product, produced by glucoamylase hydrolysis of the trisaccharide, was found to be an acceptor for alternansucrase. The disaccharide, alpha-D-glucopyranosyl-(1-->4)-L-glucose, was a better acceptor than maltose, previously the best known acceptor for alternansucrase. A structure comparison of alpha-D-glucopyranosyl-(1-->4)-L-glucose and maltose was performed through computer modeling to identify common features, which may be important in acceptor affinity by alternansucrase.lld:pubmed
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pubmed-article:15639245pubmed:authorpubmed-author:MomanyFrank...lld:pubmed
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pubmed-article:15639245pubmed:authorpubmed-author:DunlapChristo...lld:pubmed
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pubmed-article:15639245pubmed:pagination257-62lld:pubmed
pubmed-article:15639245pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:15639245pubmed:year2005lld:pubmed
pubmed-article:15639245pubmed:articleTitleAlternansucrase acceptor reactions with D-tagatose and L-glucose.lld:pubmed
pubmed-article:15639245pubmed:affiliationBioproducts and Biocatalysis Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, United States Department of Agriculture, 1815 North University Street, Peoria, IL 61604, USA. cotegl@ncaur.usda.govlld:pubmed
pubmed-article:15639245pubmed:publicationTypeJournal Articlelld:pubmed