| pubmed-article:15605243 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15605243 | lifeskim:mentions | umls-concept:C0016832 | lld:lifeskim |
| pubmed-article:15605243 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:15605243 | lifeskim:mentions | umls-concept:C0242726 | lld:lifeskim |
| pubmed-article:15605243 | lifeskim:mentions | umls-concept:C0450429 | lld:lifeskim |
| pubmed-article:15605243 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:15605243 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:15605243 | lifeskim:mentions | umls-concept:C1832072 | lld:lifeskim |
| pubmed-article:15605243 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:15605243 | pubmed:dateCreated | 2005-4-21 | lld:pubmed |
| pubmed-article:15605243 | pubmed:abstractText | alpha-Expansins are extracellular proteins that increase plant cell-wall extensibility. We analysed their pattern of expression in cucumber roots in the presence and in the absence of the mycorrhizal fungus, Glomus versiforme. The distribution of alpha-expansins was investigated by use of two polyclonal antibodies (anti-EXPA1 and anti-EXPA2, prepared against two different cucumber alpha-expansins) in immunoblotting, immunofluorescence, and immunogold experiments. Immunoblot results indicate the presence of a 30-kDa band specific for mycorrhizal roots. The two antibodies identify antigens with a different distribution in mycorrhizal roots: anti-EXPA1 labels the interface zone, but the plant cell walls only weakly. By contrast, the anti-EXPA2 labels only the plant cell walls. In order to understand the potential role of alpha-expansins during the accommodation of the fungus inside root cells, we prepared semi-thin sections to measure the size of cortical cells and the thickness of cortical cell walls in mycorrhizal and non-mycorrhizal root. Mycorrhizal cortical cells were significantly larger than non-mycorrhizal cells and had thicker cell walls. In double-labelling experiments with cellobiohydrolase-gold complex, we observed that cellulose was co-localized with alpha-expansins. Taken together, the results demonstrate that alpha-expansins are more abundant in the cucumber cell walls upon mycorrhizal infection; we propose that these wall-loosening proteins are directly involved in the accommodation of the fungus by infected cortical cells. | lld:pubmed |
| pubmed-article:15605243 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15605243 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15605243 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15605243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15605243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15605243 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15605243 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15605243 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15605243 | pubmed:issn | 0032-0935 | lld:pubmed |
| pubmed-article:15605243 | pubmed:author | pubmed-author:CosgroveD JDJ | lld:pubmed |
| pubmed-article:15605243 | pubmed:author | pubmed-author:BonfantePP | lld:pubmed |
| pubmed-article:15605243 | pubmed:author | pubmed-author:BalestriniRR | lld:pubmed |
| pubmed-article:15605243 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15605243 | pubmed:volume | 220 | lld:pubmed |
| pubmed-article:15605243 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15605243 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15605243 | pubmed:pagination | 889-99 | lld:pubmed |
| pubmed-article:15605243 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
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| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:meshHeading | pubmed-meshheading:15605243... | lld:pubmed |
| pubmed-article:15605243 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15605243 | pubmed:articleTitle | Differential location of alpha-expansin proteins during the accommodation of root cells to an arbuscular mycorrhizal fungus. | lld:pubmed |
| pubmed-article:15605243 | pubmed:affiliation | Istituto per la Protezione delle Piante del CNR, Sezione di Micologia and Dipartimento di Biologia Vegetale dell'Università, Viale Mattioli 25, 10125, Turin, Italy. | lld:pubmed |
| pubmed-article:15605243 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15605243 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15605243 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15605243 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15605243 | lld:pubmed |
