| pubmed-article:15591037 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15591037 | lifeskim:mentions | umls-concept:C1123019 | lld:lifeskim |
| pubmed-article:15591037 | lifeskim:mentions | umls-concept:C0018207 | lld:lifeskim |
| pubmed-article:15591037 | lifeskim:mentions | umls-concept:C0262950 | lld:lifeskim |
| pubmed-article:15591037 | lifeskim:mentions | umls-concept:C0441472 | lld:lifeskim |
| pubmed-article:15591037 | lifeskim:mentions | umls-concept:C1853126 | lld:lifeskim |
| pubmed-article:15591037 | lifeskim:mentions | umls-concept:C1325896 | lld:lifeskim |
| pubmed-article:15591037 | lifeskim:mentions | umls-concept:C0021469 | lld:lifeskim |
| pubmed-article:15591037 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:15591037 | pubmed:dateCreated | 2004-12-13 | lld:pubmed |
| pubmed-article:15591037 | pubmed:abstractText | We have recently reported that bone morphogenetic protein-4 (BMP-4) can inhibit progesterone production by ovine granulosa cells (GCs). Here, we have investigated the underlying mechanisms of this effect in basal as well as in FSH-induced conditions. We have confirmed that treatment with BMP-4 decreased basal GC progesterone secretion and totally abolished FSH-stimulating action. This inhibitory action was associated with a decrease in the expression of cAMP-regulated genes, steroidogenic acute regulatory protein (StAR) and P450 side-chain cleavage (P450 scc) at mRNA and protein levels. However, BMP-4 did not alter basal cAMP production by GCs. In contrast, BMP-4 decreased by half the FSH-induced cAMP production and strongly inhibited cAMP-induced progesterone production. Thus, the inhibitory effect of BMP-4 was exerted both upstream and downstream of cAMP signalling. We next examined the downstream effect, focusing on cAMP-dependent transcription factors, steroidogenic factor-1 (SF-1) and CREB, through the BMP factor signalling intermediary, Smad1. As expected, BMP-4 induced phosphorylation and transcriptional activity of Smad1 in ovine GCs. BMP-4-activated Smad1 did not affect CREB activity but inhibited the transcriptional activity of SF-1 on the canonical SF-1 responsive element. Interestingly, this transcriptional inhibitory mechanism occurred on transfected StAR and P450 scc promoter. Based on these results, we propose that SF-1 is a key target in the inhibitory mechanism exerted by BMP-4 on progesterone synthesis by ovine GCs in culture. Because SF-1 plays an essential role in the differentiation of GCs, our findings could have new implications in understanding the role of BMP family members in the control of ovarian folliculogenesis. | lld:pubmed |
| pubmed-article:15591037 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15591037 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15591037 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15591037 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:15591037 | pubmed:issn | 0952-5041 | lld:pubmed |
| pubmed-article:15591037 | pubmed:author | pubmed-author:DupontJJ | lld:pubmed |
| pubmed-article:15591037 | pubmed:author | pubmed-author:PisseletCC | lld:pubmed |
| pubmed-article:15591037 | pubmed:author | pubmed-author:FabreSS | lld:pubmed |
| pubmed-article:15591037 | pubmed:author | pubmed-author:MongetPP | lld:pubmed |
| pubmed-article:15591037 | pubmed:author | pubmed-author:MonniauxDD | lld:pubmed |
| pubmed-article:15591037 | pubmed:author | pubmed-author:PierreAA | lld:pubmed |
| pubmed-article:15591037 | pubmed:author | pubmed-author:Mandon-PépinB... | lld:pubmed |
| pubmed-article:15591037 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15591037 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:15591037 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15591037 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15591037 | pubmed:pagination | 805-17 | lld:pubmed |
| pubmed-article:15591037 | pubmed:dateRevised | 2009-8-12 | lld:pubmed |
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| pubmed-article:15591037 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15591037 | pubmed:articleTitle | Molecular basis of bone morphogenetic protein-4 inhibitory action on progesterone secretion by ovine granulosa cells. | lld:pubmed |
| pubmed-article:15591037 | pubmed:affiliation | Physiologie de la Reproduction et des Comportements, UMR 6175 INRA-CNRS-Université de Tours, Haras Nationaux, 37380 Nouzilly, France. | lld:pubmed |
| pubmed-article:15591037 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15591037 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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