| pubmed-article:15583284 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15583284 | lifeskim:mentions | umls-concept:C1265292 | lld:lifeskim |
| pubmed-article:15583284 | lifeskim:mentions | umls-concept:C0028429 | lld:lifeskim |
| pubmed-article:15583284 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:15583284 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:15583284 | lifeskim:mentions | umls-concept:C0183753 | lld:lifeskim |
| pubmed-article:15583284 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:15583284 | pubmed:issue | 12 | lld:pubmed |
| pubmed-article:15583284 | pubmed:dateCreated | 2004-12-7 | lld:pubmed |
| pubmed-article:15583284 | pubmed:abstractText | Screening for colonization with methicillin-resistant Staphylococcus aureus (MRSA) is a key aspect of infection control to limit the nosocomial spread of this organism. Current methods for the detection of MRSA in clinical microbiology laboratories, including molecularly based techniques, require a culture step and the isolation of pure colonies that result in a minimum of 20 to 24 h until a result is known. We describe a qualitative in vitro diagnostic test for the rapid detection of MRSA directly from nasal swab specimens (IDI-MRSA; Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada), based upon a real-time PCR and direct detection of MRSA via amplicon hybridization with a fluorogenic target-specific molecular beacon probe. Samples from 288 patients were analyzed for the presence of MRSA with the IDI-MRSA assay, compared to detection by either direct plating or enrichment broth selective culture methods. The diagnostic values for this MRSA screening method were 91.7% sensitivity, 93.5% specificity, 82.5% positive predictive value, and 97.1% negative predictive value when compared to culture-based methods. The time from the start of processing of specimen to result was approximately 1.5 h. In our hands, the IDI-MRSA assay is a sensitive and specific test for detection of nasal colonization with MRSA and providing for same-day results, allowing more efficient and effective use of infection control resources to control MRSA in health care facilities. | lld:pubmed |
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| pubmed-article:15583284 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15583284 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15583284 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15583284 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15583284 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:15583284 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:15583284 | pubmed:author | pubmed-author:DunneW... | lld:pubmed |
| pubmed-article:15583284 | pubmed:author | pubmed-author:WarrenDavid... | lld:pubmed |
| pubmed-article:15583284 | pubmed:author | pubmed-author:LiaoRobert... | lld:pubmed |
| pubmed-article:15583284 | pubmed:author | pubmed-author:MerzLiana RLR | lld:pubmed |
| pubmed-article:15583284 | pubmed:author | pubmed-author:EvelandMichae... | lld:pubmed |
| pubmed-article:15583284 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15583284 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:15583284 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15583284 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15583284 | pubmed:pagination | 5578-81 | lld:pubmed |
| pubmed-article:15583284 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:15583284 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15583284 | pubmed:articleTitle | Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. | lld:pubmed |
| pubmed-article:15583284 | pubmed:affiliation | Division of Infectious Diseases, Washington University School of Medicine, Campus Box 8051, 660 S. Euclid Ave., Saint Louis, MO 63110, USA. dwarren@wustl.edu | lld:pubmed |
| pubmed-article:15583284 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15583284 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15583284 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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