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pubmed-article:15581679pubmed:abstractTextTwo proteins G2a and G2b with molecular masses of approximately 24 kDa were isolated from Naja naja atra (Taiwan cobra) venom using sequential chromatography on gel filtration, ion-exchanger and reverse phase columns. The results of Edman degradation and mass analysis revealed that G2a is a cysteine-rich protein reported previously, and G2b is a novel polypeptide. CD spectra showed that the gross conformation of G2a and G2b notably differed. G2a exhibited an activity higher than that noted with G2b on inhibiting carbachol-induced muscle contraction. However, the two proteins weakly blocked muscle contraction evoked by K+. The observations that the two proteins exhibit the toxic activity in the concentration of micromolar range suggest that they are inherently weak toxins as other snake venom cysteine-rich proteins.lld:pubmed
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pubmed-article:15581679pubmed:authorpubmed-author:LinShinne-Ren...lld:pubmed
pubmed-article:15581679pubmed:authorpubmed-author:ChangLong-Sen...lld:pubmed
pubmed-article:15581679pubmed:authorpubmed-author:LiouJau-Cheng...lld:pubmed
pubmed-article:15581679pubmed:authorpubmed-author:ChengYun-Chin...lld:pubmed
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pubmed-article:15581679pubmed:volume45lld:pubmed
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pubmed-article:15581679pubmed:pagination21-5lld:pubmed
pubmed-article:15581679pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:15581679pubmed:year2005lld:pubmed
pubmed-article:15581679pubmed:articleTitlePurification and characterization of Taiwan cobra venom proteins with weak toxicity.lld:pubmed
pubmed-article:15581679pubmed:affiliationInstitute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung 804, Taiwan, ROC. lschang@mail.nsysu.edu.twlld:pubmed
pubmed-article:15581679pubmed:publicationTypeJournal Articlelld:pubmed
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pubmed-article:15581679pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed