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pubmed-article:15567409pubmed:abstractTextProgrammed ribosomal bypassing occurs in decoding phage T4 gene 60 mRNA. Half the ribosomes bypass a 50 nucleotide gap between codons 46 and 47. Peptidyl-tRNA dissociates from the "take-off" GGA, codon 46, and re-pairs to mRNA at a matched GGA "landing site" codon directly 5' of codon 47 where translation resumes. The system described here allows the contribution of peptidyl-tRNA re-pairing to be measured independently of dissociation. The matched GGA codons have been replaced by 62 other matched codons, giving a wide range of bypassing efficiencies. Codons with G or C in either or both of the first two codon positions yielded high levels of bypassing. The results are compared with those from a complementary study of non-programmed bypassing, where the combined effects of peptidyl-tRNA dissociation and reassociation were measured. The wild-type, GGA, matched codons are the most efficient in their gene 60 context in contrast to the relatively low value in the non-programmed bypassing study.lld:pubmed
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pubmed-article:15567409pubmed:pagination39-49lld:pubmed
pubmed-article:15567409pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:15567409pubmed:articleTitleP-site pairing subtleties revealed by the effects of different tRNAs on programmed translational bypassing where anticodon re-pairing to mRNA is separated from dissociation.lld:pubmed
pubmed-article:15567409pubmed:affiliationDepartment of Human Genetics, University of Utah, 15N 2030E Rm7410, Salt Lake City, UT 84112-5330, USA.lld:pubmed
pubmed-article:15567409pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15567409pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:15567409pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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