| pubmed-article:15564515 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15564515 | lifeskim:mentions | umls-concept:C1175743 | lld:lifeskim |
| pubmed-article:15564515 | lifeskim:mentions | umls-concept:C0150369 | lld:lifeskim |
| pubmed-article:15564515 | lifeskim:mentions | umls-concept:C0030946 | lld:lifeskim |
| pubmed-article:15564515 | lifeskim:mentions | umls-concept:C0813145 | lld:lifeskim |
| pubmed-article:15564515 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:15564515 | pubmed:dateCreated | 2004-11-26 | lld:pubmed |
| pubmed-article:15564515 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15564515 | pubmed:abstractText | A novel coronavirus (SCoV) is the etiological agent of severe acute respiratory syndrome. Site-specific proteolysis plays a critical role in regulating a number of cellular and viral processes. Since the main protease of SCoV, also termed 3C-like protease, is an attractive target for drug therapy, we have developed a safe, simple, and rapid genetic screen assay to monitor the activity of the SCoV 3C-like protease. This genetic system is based on the bacteriophage lambda regulatory circuit, in which the viral repressor cI is specifically cleaved to initiate the lysogenic-to-lytic switch. A specific target for the SCoV 3C-like protease, P1/P2 (SAVLQ/SGFRK), was inserted into the lambda phage cI repressor. The target specificity of the SCoV P1/P2 repressor was evaluated by coexpression of this repressor with a chemically synthesized SCoV 3C-like protease gene construct. Upon infection of Escherichia coli cells containing the two plasmids encoding the cI. SCoV P1/P2-cro and the beta-galactosidase-SCoV 3C-like protease constructs, lambda phage replicated up to 2,000-fold more efficiently than in cells that did not express the SCoV 3C-like protease. This simple and highly specific assay can be used to monitor the activity of the SCoV 3C-like protease, and it has the potential to be used for screening specific inhibitors. | lld:pubmed |
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| pubmed-article:15564515 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15564515 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15564515 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15564515 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15564515 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15564515 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:15564515 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:15564515 | pubmed:author | pubmed-author:ClotetBonaven... | lld:pubmed |
| pubmed-article:15564515 | pubmed:author | pubmed-author:PareraMariona... | lld:pubmed |
| pubmed-article:15564515 | pubmed:author | pubmed-author:MartinezMigue... | lld:pubmed |
| pubmed-article:15564515 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15564515 | pubmed:volume | 78 | lld:pubmed |
| pubmed-article:15564515 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15564515 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15564515 | pubmed:pagination | 14057-61 | lld:pubmed |
| pubmed-article:15564515 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:meshHeading | pubmed-meshheading:15564515... | lld:pubmed |
| pubmed-article:15564515 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15564515 | pubmed:articleTitle | Genetic screen for monitoring severe acute respiratory syndrome coronavirus 3C-like protease. | lld:pubmed |
| pubmed-article:15564515 | pubmed:affiliation | Fundacio irsiCaixa, Hospital Universitari Germans Trias i Pujol, 08916 Badalona, Spain. | lld:pubmed |
| pubmed-article:15564515 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15564515 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15564515 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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