| pubmed-article:15561706 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15561706 | lifeskim:mentions | umls-concept:C0525039 | lld:lifeskim |
| pubmed-article:15561706 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:15561706 | lifeskim:mentions | umls-concept:C1158530 | lld:lifeskim |
| pubmed-article:15561706 | lifeskim:mentions | umls-concept:C0252912 | lld:lifeskim |
| pubmed-article:15561706 | lifeskim:mentions | umls-concept:C0994894 | lld:lifeskim |
| pubmed-article:15561706 | lifeskim:mentions | umls-concept:C0037791 | lld:lifeskim |
| pubmed-article:15561706 | lifeskim:mentions | umls-concept:C2732140 | lld:lifeskim |
| pubmed-article:15561706 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:15561706 | pubmed:dateCreated | 2005-2-7 | lld:pubmed |
| pubmed-article:15561706 | pubmed:abstractText | DNA polymerase beta (pol beta) and flap endonuclease 1 (FEN1) are key players in pol beta-mediated long-patch base excision repair (LP-BER). It was proposed that this type of LP-BER is accomplished through FEN1 removal of a 2- to 11-nucleotide flap created by pol beta strand displacement DNA synthesis. To understand how these enzymes might cooperate during LP-BER, we characterized purified human pol beta DNA synthesis by utilizing various BER intermediates, including single-nucleotide-gapped DNA, nicked DNA, and nicked DNA with various lengths of flaps all with a 5'-terminal tetrahydrofuran (THF) residue. We observed that nicked DNA and nicked-THF flap DNA were poor substrates for pol beta-mediated DNA synthesis; yet, DNA synthesis was strongly stimulated by purified human FEN1. FEN1 did not improve pol beta substrate binding. FEN1 cleavage activity was required for the stimulation, suggesting that FEN1 removed a barrier to pol beta DNA synthesis. In addition, FEN1 cleavage on both nicked and nicked-THF flap DNA resulted in a one-nucleotide gapped DNA molecule that was an ideal substrate for pol beta. This study demonstrates that pol beta cooperates with FEN1 to remove DNA damage via a "Hit and Run" mechanism, involving alternating short gap production by FEN1 and gap filling by pol beta, rather than through coordinated formation and removal of a strand-displaced flap. | lld:pubmed |
| pubmed-article:15561706 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15561706 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15561706 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15561706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15561706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15561706 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15561706 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15561706 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:15561706 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:15561706 | pubmed:author | pubmed-author:PrasadRajendr... | lld:pubmed |
| pubmed-article:15561706 | pubmed:author | pubmed-author:WilsonSamuel... | lld:pubmed |
| pubmed-article:15561706 | pubmed:author | pubmed-author:BeardWilliam... | lld:pubmed |
| pubmed-article:15561706 | pubmed:author | pubmed-author:ShockDavid... | lld:pubmed |
| pubmed-article:15561706 | pubmed:author | pubmed-author:LiuYuanY | lld:pubmed |
| pubmed-article:15561706 | pubmed:author | pubmed-author:HouEsther WEW | lld:pubmed |
| pubmed-article:15561706 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15561706 | pubmed:day | 4 | lld:pubmed |
| pubmed-article:15561706 | pubmed:volume | 280 | lld:pubmed |
| pubmed-article:15561706 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15561706 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15561706 | pubmed:pagination | 3665-74 | lld:pubmed |
| pubmed-article:15561706 | pubmed:meshHeading | pubmed-meshheading:15561706... | lld:pubmed |
| pubmed-article:15561706 | pubmed:meshHeading | pubmed-meshheading:15561706... | lld:pubmed |
| pubmed-article:15561706 | pubmed:meshHeading | pubmed-meshheading:15561706... | lld:pubmed |
| pubmed-article:15561706 | pubmed:meshHeading | pubmed-meshheading:15561706... | lld:pubmed |
| pubmed-article:15561706 | pubmed:meshHeading | pubmed-meshheading:15561706... | lld:pubmed |
| pubmed-article:15561706 | pubmed:meshHeading | pubmed-meshheading:15561706... | lld:pubmed |
| pubmed-article:15561706 | pubmed:meshHeading | pubmed-meshheading:15561706... | lld:pubmed |
| pubmed-article:15561706 | pubmed:year | 2005 | lld:pubmed |
| pubmed-article:15561706 | pubmed:articleTitle | DNA polymerase beta and flap endonuclease 1 enzymatic specificities sustain DNA synthesis for long patch base excision repair. | lld:pubmed |
| pubmed-article:15561706 | pubmed:affiliation | Laboratory of Structural Biology, NIEHS, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA. | lld:pubmed |
| pubmed-article:15561706 | pubmed:publicationType | Journal Article | lld:pubmed |
| entrez-gene:2237 | entrezgene:pubmed | pubmed-article:15561706 | lld:entrezgene |
| entrez-gene:5423 | entrezgene:pubmed | pubmed-article:15561706 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:15561706 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:15561706 | lld:entrezgene |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15561706 | lld:pubmed |
