| pubmed-article:15528675 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C0038409 | lld:lifeskim |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C0449943 | lld:lifeskim |
| pubmed-article:15528675 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:15528675 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:15528675 | pubmed:dateCreated | 2004-11-5 | lld:pubmed |
| pubmed-article:15528675 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15528675 | pubmed:abstractText | In a previous study, we isolated and characterized a new serotype k of Streptococcus mutans from human blood and oral cavities. Analysis of the genes involved in biosynthesis of the serotype-specific polysaccharide of serotype k strains revealed that the serotype k-specific nucleotide alignment was commonly present in the 5' region of the rgpF gene (350 bp from the initial sequence) compared to the reference strains, and then a method for rapid identification of serotype k strains was developed by use of PCR with primers designed on the basis of the sequence of the variable region. PCR assays with primers specific for amplification of serotype k strains showed a negative reaction with serotype c, e, and f strains and a positive reaction with serotype k strains, with the sensitivity for identification of the serotype k strains shown to range from 5 to 50 cells. Next, the frequency of positive reactions for serotype k-specific primers was surveyed with DNA taken from saliva samples from 200 subjects (2 to 18 years of age), and 10 of those showed a positive reaction, which was higher than the frequency in our previous survey with a serological method. In addition, all saliva samples from subjects with serotype k strains in our previous study were shown to be positive with the serotype k-specific primers. These results indicate that this new PCR method is effective for identification of subjects with S. mutans serotype k. | lld:pubmed |
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| pubmed-article:15528675 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15528675 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15528675 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15528675 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15528675 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:15528675 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:15528675 | pubmed:author | pubmed-author:NakagawaIchir... | lld:pubmed |
| pubmed-article:15528675 | pubmed:author | pubmed-author:HamadaShigeyu... | lld:pubmed |
| pubmed-article:15528675 | pubmed:author | pubmed-author:OoshimaTakash... | lld:pubmed |
| pubmed-article:15528675 | pubmed:author | pubmed-author:ShimizuNoriko... | lld:pubmed |
| pubmed-article:15528675 | pubmed:author | pubmed-author:NakanoKazuhik... | lld:pubmed |
| pubmed-article:15528675 | pubmed:author | pubmed-author:NomuraRyotaR | lld:pubmed |
| pubmed-article:15528675 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15528675 | pubmed:volume | 42 | lld:pubmed |
| pubmed-article:15528675 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15528675 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15528675 | pubmed:pagination | 4925-30 | lld:pubmed |
| pubmed-article:15528675 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:15528675 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15528675 | pubmed:articleTitle | Development of a PCR method for rapid identification of new Streptococcus mutans serotype k strains. | lld:pubmed |
| pubmed-article:15528675 | pubmed:affiliation | Department of Pediatric Dentistry, Osaka University Graduate School of Dentistry, 1-8 Yamada-oka, Suita, Osaka 565-0871, Japan. | lld:pubmed |
| pubmed-article:15528675 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15528675 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| pubmed-article:15528675 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
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