| pubmed-article:15517972 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15517972 | lifeskim:mentions | umls-concept:C0162867 | lld:lifeskim |
| pubmed-article:15517972 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:15517972 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:15517972 | lifeskim:mentions | umls-concept:C0036681 | lld:lifeskim |
| pubmed-article:15517972 | lifeskim:mentions | umls-concept:C0013855 | lld:lifeskim |
| pubmed-article:15517972 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15517972 | pubmed:dateCreated | 2004-11-2 | lld:pubmed |
| pubmed-article:15517972 | pubmed:abstractText | Current DNA electrophoretic solutions employ high ionic concentrations and require long electrophoretic run times. Here we demonstrate that high and low molecular weight double-stranded DNA, single-stranded DNA (ssDNA), and RNA can be separated rapidly in agarose-based low-molarity conductive media. Separation of small DNA fragments was optimized by substituting 1-mM solutions of alkali metals or a nonbiological amine that distributed voltage with a minute current. These ultra-dilute solutions can separate DNA at least 15-fold faster Low-molarity media at 5-10 mM adequately separated RNA and larger DNA fragments as well. These novel media reduce the Joule heating of the electrophoretic system and allow for easy-to-use, ultra-fast separation of DNA fragments. | lld:pubmed |
| pubmed-article:15517972 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15517972 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15517972 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15517972 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15517972 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15517972 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15517972 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15517972 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15517972 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15517972 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15517972 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:15517972 | pubmed:issn | 0736-6205 | lld:pubmed |
| pubmed-article:15517972 | pubmed:author | pubmed-author:KernScott ESE | lld:pubmed |
| pubmed-article:15517972 | pubmed:author | pubmed-author:CalhounEric... | lld:pubmed |
| pubmed-article:15517972 | pubmed:author | pubmed-author:GallmeierEike... | lld:pubmed |
| pubmed-article:15517972 | pubmed:author | pubmed-author:BrodyJonathan... | lld:pubmed |
| pubmed-article:15517972 | pubmed:author | pubmed-author:CreavalleTali... | lld:pubmed |
| pubmed-article:15517972 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15517972 | pubmed:volume | 37 | lld:pubmed |
| pubmed-article:15517972 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15517972 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15517972 | pubmed:pagination | 598, 600, 602 | lld:pubmed |
| pubmed-article:15517972 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:meshHeading | pubmed-meshheading:15517972... | lld:pubmed |
| pubmed-article:15517972 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15517972 | pubmed:articleTitle | Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. | lld:pubmed |
| pubmed-article:15517972 | pubmed:affiliation | The Johns Hopkins University School of Medicine, Baltimore, MD 21231, USA. sk@jhmi.edu | lld:pubmed |
| pubmed-article:15517972 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15517972 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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