| pubmed-article:1550339 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0032105 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0042036 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0205103 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C1306673 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0024495 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0332120 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0047359 | lld:lifeskim |
| pubmed-article:1550339 | lifeskim:mentions | umls-concept:C0165830 | lld:lifeskim |
| pubmed-article:1550339 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1550339 | pubmed:dateCreated | 1992-4-17 | lld:pubmed |
| pubmed-article:1550339 | pubmed:abstractText | 3-Deoxyglucose (3-deoxy-D-erythro-hexos-2-ulose) (3-DG) is a reactive dicarbonyl intermediate involved in the polymerization and browning of proteins by glucose in vitro. Damage to protein by formation of 3-DG in vivo is thought to be limited by enzymes which convert 3-DG to less reactive species, such as 3-deoxyfructose (3-DF). We have developed a sensitive and specific assay for measuring 3-DG and 3-DF in human urine and plasma. In this assay, 3-DG and 3-DF are reduced to 3-deoxy-hexitols (3-DH), using either NaBH4 or NaBD4, and then analyzed by selected ion monitoring gas chromatography-mass spectrometry. Based on comparative analysis of samples reduced with NaBD4 versus NaBD4, 3-DH in urine was derived exclusively (greater than 99%) from 3-DF, while 3-DG accounted for approximately 15% of 3-DH in plasma. The concentrations of 3-DH in fasting human urine and plasma were 5.3 +/- 1.5 micrograms/mg creatinine (n = 18) and 7.2 +/- 1.7 micrograms/dl (n = 18), respectively. The concentrations of 3-DG and 3-DF in plasma (n = 7) were 1.0 +/- 0.2 and 6.7 +/- 1.6 micrograms/dl, respectively. These results suggest that several milligrams of 3-DG are formed in the body per day and detoxified by reduction to 3-DF and support the role of 3-DG as an intermediate in the browning of protein via the Maillard reaction in vivo. | lld:pubmed |
| pubmed-article:1550339 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1550339 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1550339 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1550339 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:1550339 | pubmed:issn | 0003-9861 | lld:pubmed |
| pubmed-article:1550339 | pubmed:author | pubmed-author:BaynesJ WJW | lld:pubmed |
| pubmed-article:1550339 | pubmed:author | pubmed-author:FeatherM SMS | lld:pubmed |
| pubmed-article:1550339 | pubmed:author | pubmed-author:KnechtK JKJ | lld:pubmed |
| pubmed-article:1550339 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1550339 | pubmed:volume | 294 | lld:pubmed |
| pubmed-article:1550339 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1550339 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1550339 | pubmed:pagination | 130-7 | lld:pubmed |
| pubmed-article:1550339 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:meshHeading | pubmed-meshheading:1550339-... | lld:pubmed |
| pubmed-article:1550339 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1550339 | pubmed:articleTitle | Detection of 3-deoxyfructose and 3-deoxyglucosone in human urine and plasma: evidence for intermediate stages of the Maillard reaction in vivo. | lld:pubmed |
| pubmed-article:1550339 | pubmed:affiliation | Department of Chemistry, University of South Carolina, Columbia 29208. | lld:pubmed |
| pubmed-article:1550339 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1550339 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1550339 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1550339 | lld:pubmed |
