| pubmed-article:1548980 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1548980 | lifeskim:mentions | umls-concept:C0024109 | lld:lifeskim |
| pubmed-article:1548980 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:1548980 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:1548980 | lifeskim:mentions | umls-concept:C0025255 | lld:lifeskim |
| pubmed-article:1548980 | lifeskim:mentions | umls-concept:C2745878 | lld:lifeskim |
| pubmed-article:1548980 | lifeskim:mentions | umls-concept:C0016349 | lld:lifeskim |
| pubmed-article:1548980 | pubmed:issue | 13 | lld:pubmed |
| pubmed-article:1548980 | pubmed:dateCreated | 1992-4-17 | lld:pubmed |
| pubmed-article:1548980 | pubmed:abstractText | An enzyme partially purified from bovine lung membranes appears to be endothelin converting enzyme (ECE). This enzyme specifically cleaves big endothelin-1 (big ET-1) at the proper site, between Trp21 and Val22, with maximum activity at pH 7.5 and with a Km of roughly 3 microM, to produce endothelin-1 (ET-1) and C-terminal peptide (CTP). This same enzyme hydrolyzes the fluorogenic substrate succinyl-Ile-Ile-Trp-methylcoumarinamide to release the highly fluorescent 7-amino-4-methylcoumarin. The peptide derivative has the same amino acid sequence as big ET-1 and is a good substrate with a Km of about 27 microM. This enzyme is a metalloproteinase. It is not inhibited by five common proteinase inhibitors (pepstatin A, PMSF, NEM, E-64 and thiorphan) but it is inhibited by phosphoramidon and chelating compounds. The apoenzyme is restored to nearly full activity by a zinc-EDTA buffer with pZn = 13. | lld:pubmed |
| pubmed-article:1548980 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1548980 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1548980 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1548980 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:1548980 | pubmed:author | pubmed-author:WilsonI BIB | lld:pubmed |
| pubmed-article:1548980 | pubmed:author | pubmed-author:KunduG CGC | lld:pubmed |
| pubmed-article:1548980 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1548980 | pubmed:volume | 50 | lld:pubmed |
| pubmed-article:1548980 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1548980 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1548980 | pubmed:pagination | 965-70 | lld:pubmed |
| pubmed-article:1548980 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:1548980 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1548980 | pubmed:articleTitle | Identification of endothelin converting enzyme in bovine lung membranes using a new fluorogenic substrate. | lld:pubmed |
| pubmed-article:1548980 | pubmed:affiliation | Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215. | lld:pubmed |
| pubmed-article:1548980 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1548980 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1548980 | lld:pubmed |
