| pubmed-article:15478796 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15478796 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:15478796 | lifeskim:mentions | umls-concept:C0035647 | lld:lifeskim |
| pubmed-article:15478796 | lifeskim:mentions | umls-concept:C0022917 | lld:lifeskim |
| pubmed-article:15478796 | lifeskim:mentions | umls-concept:C0276852 | lld:lifeskim |
| pubmed-article:15478796 | lifeskim:mentions | umls-concept:C0004573 | lld:lifeskim |
| pubmed-article:15478796 | lifeskim:mentions | umls-concept:C1521840 | lld:lifeskim |
| pubmed-article:15478796 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:15478796 | pubmed:dateCreated | 2004-10-13 | lld:pubmed |
| pubmed-article:15478796 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:abstractText | In this study, we characterized a novel Babesia bovis cDNA clone obtained by immunoscreening the cDNA expression phage library with B. bovis-infected bovine serum. The genetic analyses showed that it contained an open reading frame of 993 bp, which was considered to encode B. bovis L-lactate dehydrogenase (BbLDH: E.C. 1.1.1.27) because of the strikingly high amino acid identities of its gene product to the LDHs of Plasmodium falciparum and Toxoplasma gondii. Immunological analyses with the anti-recombinant BbLDH mouse serum showed that 36 kDa of the native BbLDH was expressed not only in the cytoplasm of intra- and extraerythrocytic parasites but also along the membrane of infected erythrocytes. The kinetic properties of recombinant BbLDH proved a certain enzymatic activity of LDH, and the activity was significantly inhibited by the addition of gossypol, a competitive inhibitor of protozoan LDHs. Moreover, 100 microM of the gossypol irretrievably arrested the in vitro growth of B. bovis. The results demonstrated that BbLDH provides a suitable drug target for the design of novel babesial chemotherapeutics. | lld:pubmed |
| pubmed-article:15478796 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15478796 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15478796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15478796 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15478796 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:15478796 | pubmed:issn | 0166-6851 | lld:pubmed |
| pubmed-article:15478796 | pubmed:author | pubmed-author:HirataHaruyuk... | lld:pubmed |
| pubmed-article:15478796 | pubmed:author | pubmed-author:YokoyamaNaoak... | lld:pubmed |
| pubmed-article:15478796 | pubmed:author | pubmed-author:IgarashiIkuoI | lld:pubmed |
| pubmed-article:15478796 | pubmed:author | pubmed-author:SuthisakBoonc... | lld:pubmed |
| pubmed-article:15478796 | pubmed:author | pubmed-author:BorkSabineS | lld:pubmed |
| pubmed-article:15478796 | pubmed:author | pubmed-author:OkamuraMasash... | lld:pubmed |
| pubmed-article:15478796 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15478796 | pubmed:volume | 136 | lld:pubmed |
| pubmed-article:15478796 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15478796 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15478796 | pubmed:pagination | 165-72 | lld:pubmed |
| pubmed-article:15478796 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15478796 | pubmed:meshHeading | pubmed-meshheading:15478796... | lld:pubmed |
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| pubmed-article:15478796 | pubmed:meshHeading | pubmed-meshheading:15478796... | lld:pubmed |
| pubmed-article:15478796 | pubmed:meshHeading | pubmed-meshheading:15478796... | lld:pubmed |
| pubmed-article:15478796 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15478796 | pubmed:articleTitle | Identification of Babesia bovis L-lactate dehydrogenase as a potential chemotherapeutical target against bovine babesiosis. | lld:pubmed |
| pubmed-article:15478796 | pubmed:affiliation | National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. | lld:pubmed |
| pubmed-article:15478796 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15478796 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:15478796 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
