| pubmed-article:1547812 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C0733755 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C0596988 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C2003941 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C1511636 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C0205349 | lld:lifeskim |
| pubmed-article:1547812 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:1547812 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:1547812 | pubmed:dateCreated | 1992-4-21 | lld:pubmed |
| pubmed-article:1547812 | pubmed:abstractText | To study the structural basis for specificity and affinity of cytotoxic T lymphocytes for major histocompatibility complex/peptide complexes, we have employed a cytotoxic T lymphocyte (CTL)-mediated immunoselection approach to obtain H-2Kb structural mutants which are resistant to lysis by a Kb-specific alloreactive CTL clone. In this study we describe the Kb structural mutant, designated R8.60.14, recovered following immunoselection using the CD8-dependent CTL clone 60 as a selective agent. Although serologically unaltered with respect to Kb expression, R8.60.14 is not recognized by CD8-dependent, Kb-specific CTL. DNA sequence analysis revealed a single Glu----Lys amino acid substitution at position 222 in the Kb alpha 3-domain of this variant. To determine if a direct correlation exists between CD8 dependence of a Kb specific CTL and its failure to respond to R8.60.14, we examined the lytic response against R8.60.14 by CD8-independent, Kb-specific CTL obtained from long-term culture in the presence of anti-CD8 monoclonal antibody, 3.155. CD8-independent CTL exhibit no difference in their response against the R8 parent and R8.60.14 variant. This study demonstrates unequivocally that Kb-specific recognition of R8.60.14 by CD8-independent CTL is unaltered, while the response by CD8-dependent CTL is completely abrogated. Thus, the sole basis for emergence of this variant in the CTL-mediated immunoselection approach used in this study resides in the alteration of a single CD8-binding site residue at position 222 in the Kb alpha 3 domain. The functional importance of this Glu222 residue for the interaction between the CD8 molecule on CD8-dependent CTL and the Kb alpha 3 domain is further reinforced by virtue of the recovery of the R8.60.14 variant on the basis of its resistance to lysis by a CD8-dependent CTL clone in this CTL-mediated immunoselection approach. | lld:pubmed |
| pubmed-article:1547812 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1547812 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1547812 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1547812 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1547812 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1547812 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1547812 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1547812 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1547812 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1547812 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1547812 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:1547812 | pubmed:issn | 0014-2980 | lld:pubmed |
| pubmed-article:1547812 | pubmed:author | pubmed-author:SheilJ MJM | lld:pubmed |
| pubmed-article:1547812 | pubmed:author | pubmed-author:NathensonS... | lld:pubmed |
| pubmed-article:1547812 | pubmed:author | pubmed-author:SunRR | lld:pubmed |
| pubmed-article:1547812 | pubmed:author | pubmed-author:ShepherdS ESE | lld:pubmed |
| pubmed-article:1547812 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1547812 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:1547812 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1547812 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1547812 | pubmed:pagination | 647-53 | lld:pubmed |
| pubmed-article:1547812 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:meshHeading | pubmed-meshheading:1547812-... | lld:pubmed |
| pubmed-article:1547812 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1547812 | pubmed:articleTitle | Selective reactivity of CD8-independent T lymphocytes to a cytotoxic T lymphocyte-selected H-2Kb mutant altered at position 222 in the alpha 3 domain. | lld:pubmed |
| pubmed-article:1547812 | pubmed:affiliation | Department of Microbiology, West Virginia University Health Sciences Center, Morgantown 26506. | lld:pubmed |
| pubmed-article:1547812 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1547812 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1547812 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1547812 | lld:pubmed |
