| pubmed-article:15456223 | pubmed:abstractText | A preparation with fructose-1,6-bisphosphatase (FBPase) activity has been isolated as a result of the five-stage treatment of the culture liquid obtained after growing the agent of pale-green dwarfness of cereals on the medium CM IMB-72. After this enzyme treatment by means of hydrophobic chromatography on the column from Toyopearl HW-60 (the enzyme was obtained from the column with decrease of ammonium sulphate (AS) concentration in the eluating buffer to 0.8 M), the preparation deprivation of AS on the column with Sephadex G-10 and substrate-dependent chromatography on the column with CM-sepharose the extracellular 176-fold purified FBPase. Acholeplasma laidlawii var. granulum strain 118 was obtained--the main pathogenicity factor for the agent of cereals yellow. | lld:pubmed |
