| pubmed-article:15450817 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15450817 | lifeskim:mentions | umls-concept:C0035647 | lld:lifeskim |
| pubmed-article:15450817 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:15450817 | lifeskim:mentions | umls-concept:C0003209 | lld:lifeskim |
| pubmed-article:15450817 | lifeskim:mentions | umls-concept:C0220908 | lld:lifeskim |
| pubmed-article:15450817 | lifeskim:mentions | umls-concept:C1150066 | lld:lifeskim |
| pubmed-article:15450817 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:15450817 | pubmed:dateCreated | 2004-9-28 | lld:pubmed |
| pubmed-article:15450817 | pubmed:abstractText | Heparanase plays an important role in the degradation of the extracellular matrix. It is implicated in inflammation, tumor angiogenesis and metastasis. We have developed two high-throughput methods for measuring heparanase activity and screening potential inhibitors. The first method involves coating fibroblast growth factor (FGF) on microtiter plates and capturing fluorescein isothiocyanate (FITC)-labeled heparin sulfate (HS), which is used as a substrate for heparanase digestion. Labeled HS fragments are released into the medium and quantitated by fluorescence intensity measurement. We have implemented this assay method into a Zeiss uHTS system and screened compound libraries for heparanase inhibitors. The second method involves labeling HS with biotin followed by FITC to generate a dual-labeled HS. The labeled material is bound to streptavidin-coated plates and used as a substrate for heparanase digestion. Both methods are sensitive and easily applicable to robotic systems. In addition, we have labeled both HS and biotin-HS with Eu-chelate, a fluorophore that exhibits long decay fluorescence. Assays using Eu-labeled HS and Eu-labeled biotin-HS have been developed and show higher sensitivity than those using FITC-labeled material. Furthermore, assays using Eu-chelate HS (or biotin-HS) should eliminate the interference of fluorescence compounds. | lld:pubmed |
| pubmed-article:15450817 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15450817 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15450817 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15450817 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:15450817 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:15450817 | pubmed:author | pubmed-author:RobertsJohnJ | lld:pubmed |
| pubmed-article:15450817 | pubmed:author | pubmed-author:LevinWayneW | lld:pubmed |
| pubmed-article:15450817 | pubmed:author | pubmed-author:HuangKuo-SenK... | lld:pubmed |
| pubmed-article:15450817 | pubmed:author | pubmed-author:LiuChao-MinCM | lld:pubmed |
| pubmed-article:15450817 | pubmed:author | pubmed-author:HolmgrenJanna... | lld:pubmed |
| pubmed-article:15450817 | pubmed:author | pubmed-author:ReikLindaL | lld:pubmed |
| pubmed-article:15450817 | pubmed:author | pubmed-author:Lucas-McGadyD... | lld:pubmed |
| pubmed-article:15450817 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15450817 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:15450817 | pubmed:volume | 333 | lld:pubmed |
| pubmed-article:15450817 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15450817 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15450817 | pubmed:pagination | 389-98 | lld:pubmed |
| pubmed-article:15450817 | pubmed:dateRevised | 2005-11-17 | lld:pubmed |
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| pubmed-article:15450817 | pubmed:meshHeading | pubmed-meshheading:15450817... | lld:pubmed |
| pubmed-article:15450817 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15450817 | pubmed:articleTitle | High-throughput methods for measuring heparanase activity and screening potential antimetastatic and anti-inflammatory agents. | lld:pubmed |
| pubmed-article:15450817 | pubmed:affiliation | Department of Discovery Technologies, Hoffmann-La Roche Inc., 340 Kingsland Street, Nutley, NJ 07110, USA. kuo-sen.huang@roche.com | lld:pubmed |
| pubmed-article:15450817 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15450817 | lld:pubmed |
