| pubmed-article:1537432 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1537432 | lifeskim:mentions | umls-concept:C0015731 | lld:lifeskim |
| pubmed-article:1537432 | lifeskim:mentions | umls-concept:C0282587 | lld:lifeskim |
| pubmed-article:1537432 | lifeskim:mentions | umls-concept:C0205419 | lld:lifeskim |
| pubmed-article:1537432 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:1537432 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:1537432 | pubmed:dateCreated | 1992-4-1 | lld:pubmed |
| pubmed-article:1537432 | pubmed:abstractText | The neural cell adhesion molecule (N-CAM) is expressed in a specific spatiotemporal pattern during feather development, suggesting that adhesion mediated by this molecule is involved in feather morphogenesis. To begin to investigate N-CAM's function in developing feathers, we determined what forms of N-CAM polypeptide are present and the distribution of polysialic acid (PSA), a carbohydrate moiety that decreases N-CAM-mediated cellular adhesion. N-CAM in skin appears as a Mr 145-kDa polypeptide compared to the 140-kDa brain N-CAM polypeptide, and is encoded by a 6.4-kb mRNA, compared to the 6.1-kb mRNA in brain. Polymerase chain reaction analysis of the exon splicing pattern of skin N-CAM shows that the 6.4-kb mRNA band represents two transcripts, with and without a 93-bp insert between exons 12 and 13. Thus, two N-CAM polypeptides are expressed in skin, but the 93-bp insert does not account for the larger size of the skin mRNAs and polypeptides. We show that the size difference of the polypeptides is instead due to N-linked oligosaccharides attached to the skin N-CAM proteins. The larger size of the skin mRNAs may be due to use of a different transcriptional start site. Staining of skin sections and wholemounts confirms previous descriptions of N-CAM in developing feathers, but reveals that N-CAM is also present at low levels on epidermal cells as early as stage 29 (E6). We find that PSA is expressed only on a subset of the cells that express N-CAM, in particular on dermal cells in the feather rudiments from stage 35-36 (E9-10) and on smooth muscle cells at the base of the filaments from stage 37 (E11) until the latest stage examined (stage 44, E18). The known effects on cell-cell adhesion of amount of N-CAM and PSA suggest that the variations we observe in skin may regulate cell-cell interactions that are important in feather development. | lld:pubmed |
| pubmed-article:1537432 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1537432 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1537432 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1537432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1537432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1537432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1537432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1537432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1537432 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1537432 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1537432 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:1537432 | pubmed:issn | 0012-1606 | lld:pubmed |
| pubmed-article:1537432 | pubmed:author | pubmed-author:GallinW JWJ | lld:pubmed |
| pubmed-article:1537432 | pubmed:author | pubmed-author:MarshR GRG | lld:pubmed |
| pubmed-article:1537432 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1537432 | pubmed:volume | 150 | lld:pubmed |
| pubmed-article:1537432 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1537432 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1537432 | pubmed:pagination | 171-84 | lld:pubmed |
| pubmed-article:1537432 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1537432 | pubmed:meshHeading | pubmed-meshheading:1537432-... | lld:pubmed |
| pubmed-article:1537432 | pubmed:meshHeading | pubmed-meshheading:1537432-... | lld:pubmed |
| pubmed-article:1537432 | pubmed:meshHeading | pubmed-meshheading:1537432-... | lld:pubmed |
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| pubmed-article:1537432 | pubmed:meshHeading | pubmed-meshheading:1537432-... | lld:pubmed |
| pubmed-article:1537432 | pubmed:meshHeading | pubmed-meshheading:1537432-... | lld:pubmed |
| pubmed-article:1537432 | pubmed:meshHeading | pubmed-meshheading:1537432-... | lld:pubmed |
| pubmed-article:1537432 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1537432 | pubmed:articleTitle | Structural variants of the neural cell adhesion molecule (N-CAM) in developing feathers. | lld:pubmed |
| pubmed-article:1537432 | pubmed:affiliation | Department of Zoology, University of Alberta, Edmonton, Canada. | lld:pubmed |
| pubmed-article:1537432 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1537432 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1537432 | lld:pubmed |
