| pubmed-article:15363161 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15363161 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15363161 | lifeskim:mentions | umls-concept:C0527443 | lld:lifeskim |
| pubmed-article:15363161 | lifeskim:mentions | umls-concept:C0029433 | lld:lifeskim |
| pubmed-article:15363161 | lifeskim:mentions | umls-concept:C0205223 | lld:lifeskim |
| pubmed-article:15363161 | lifeskim:mentions | umls-concept:C0032521 | lld:lifeskim |
| pubmed-article:15363161 | pubmed:issue | 7-8 | lld:pubmed |
| pubmed-article:15363161 | pubmed:dateCreated | 2004-9-14 | lld:pubmed |
| pubmed-article:15363161 | pubmed:abstractText | The ability to deliver, over time, biologically active osteogenic growth factors by means of designed scaffolds to sites of tissue regeneration offers tremendous therapeutic opportunities in a variety of musculoskeletal diseases. The aims of this study were to generate porous biodegradable scaffolds encapsulating an osteogenic protein, bone morphogenetic protein 2 (BMP-2), and to examine the ability of the scaffolds to promote human osteoprogenitor differentiation and bone formation in vitro and in vivo. BMP-2-encapsulated poly(DL-lactic acid) (PLA) scaffolds were generated by an innovative supercritical fluid process developed for solvent-sensitive and thermolabile growth factors. BMP-2 released from encapsulated constructs promoted adhesion, migration, expansion, and differentiation of human osteoprogenitor cells on three-dimensional scaffolds. Enhanced matrix synthesis and cell differentiation on growth factor-encapsulated scaffolds was observed after culture in an ex vivo model of bone formation developed on the basis of the chick chorioallantoic membrane model. BMP-2-encapsulated polymer scaffolds showed morphologic evidence of new bone matrix and cartilage formation after subcutaneous implantation and within diffusion chambers implanted into athymic mice as assessed by X-ray analysis and immunocytochemistry. The generation of three-dimensional biomimetic structures incorporating osteoinductive factors such as BMP-2 indicates their potential for de novo bone formation that exploits cell-matrix interactions and, significantly, realistic delivery protocols for growth factors in musculoskeletal tissue engineering. | lld:pubmed |
| pubmed-article:15363161 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15363161 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15363161 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15363161 | pubmed:issn | 1076-3279 | lld:pubmed |
| pubmed-article:15363161 | pubmed:author | pubmed-author:SebaldWalterW | lld:pubmed |
| pubmed-article:15363161 | pubmed:author | pubmed-author:OreffoRichard... | lld:pubmed |
| pubmed-article:15363161 | pubmed:author | pubmed-author:HowdleSteven... | lld:pubmed |
| pubmed-article:15363161 | pubmed:author | pubmed-author:ShakesheffKev... | lld:pubmed |
| pubmed-article:15363161 | pubmed:author | pubmed-author:ClarkeNichola... | lld:pubmed |
| pubmed-article:15363161 | pubmed:author | pubmed-author:WhitakerMarti... | lld:pubmed |
| pubmed-article:15363161 | pubmed:author | pubmed-author:YangXuebin... | lld:pubmed |
| pubmed-article:15363161 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15363161 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:15363161 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15363161 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15363161 | pubmed:pagination | 1037-45 | lld:pubmed |
| pubmed-article:15363161 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:15363161 | pubmed:articleTitle | Human osteoprogenitor bone formation using encapsulated bone morphogenetic protein 2 in porous polymer scaffolds. | lld:pubmed |
| pubmed-article:15363161 | pubmed:affiliation | University Orthopaedics, University of Southampton, General Hospital, Southampton S016 6YD, UK. | lld:pubmed |
| pubmed-article:15363161 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15363161 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15363161 | lld:pubmed |
