| pubmed-article:15350831 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15350831 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15350831 | lifeskim:mentions | umls-concept:C0376358 | lld:lifeskim |
| pubmed-article:15350831 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:15350831 | lifeskim:mentions | umls-concept:C0596402 | lld:lifeskim |
| pubmed-article:15350831 | lifeskim:mentions | umls-concept:C1832073 | lld:lifeskim |
| pubmed-article:15350831 | lifeskim:mentions | umls-concept:C0596235 | lld:lifeskim |
| pubmed-article:15350831 | pubmed:issue | 19 | lld:pubmed |
| pubmed-article:15350831 | pubmed:dateCreated | 2004-9-7 | lld:pubmed |
| pubmed-article:15350831 | pubmed:abstractText | The effect of nordihydroguaiaretic acid (NDGA), a compound commonly used as a lipoxygenases inhibitor, on intracellular free Ca2+ levels ([Ca2+]i) in PC3 human prostate cancer cells was investigated. [Ca2+]i was measured by using the Ca2+ -sensitive dye fura-2. NDGA increased [Ca2+]i in a concentration-dependent manner with an EC50 of 30 microM. The Ca2+ signal comprised a gradual and sustained increase. Removal of extracellular Ca2+ partly decreased the NDGA-induced [Ca2+]i increase, suggesting that the Ca2+ signal was due to both extracellular Ca2+ influx and intracellular Ca2+ release. NDGA-induced Ca2+ influx was independently confirmed by measuring NDGA-induced Mn2+ -coupled quench of fura-2 fluorescence. The NDGA-induced Ca2+ influx was not affected by L-type Ca2+ channel blockers. In Ca2+ -free medium, the NDGA-induced [Ca2+]i increase was abolished by pretreatment with 1 microM thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor), and conversely, pretreatment with NDGA abolished thapsigargin-induced [Ca2+]i increase. NDGA-induced intracellular Ca2+ release was not altered by inhibition of phospholipase C. Overnight treatment with 20-50 microM NDGA inhibited cell proliferation rate in a concentration-dependent manner. Several other lipoxygenases inhibitors did not alter [Ca2+]i. Collectively, this study shows that in prostate cells, NDGA induced a [Ca2+]i increase via releasing stored Ca2+ from the endoplasmic reticulum in a manner independent of phospholipase C activity, and by causing Ca2+ influx. NDGA also caused cytotoxicity at higher concentrations. | lld:pubmed |
| pubmed-article:15350831 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15350831 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15350831 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15350831 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15350831 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15350831 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:15350831 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15350831 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15350831 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:15350831 | pubmed:issn | 0024-3205 | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:ChangHong-Tai... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:JanChung-RenC... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:ChenWei-Chuan... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:HuangJong-Khi... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:HuangChun-Jen... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:JiannBang-Pin... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:ChenJin-ShyrJ... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:ChengHe-Hsiun... | lld:pubmed |
| pubmed-article:15350831 | pubmed:author | pubmed-author:HsuShu-ShongS... | lld:pubmed |
| pubmed-article:15350831 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15350831 | pubmed:day | 24 | lld:pubmed |
| pubmed-article:15350831 | pubmed:volume | 75 | lld:pubmed |
| pubmed-article:15350831 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15350831 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15350831 | pubmed:pagination | 2341-51 | lld:pubmed |
| pubmed-article:15350831 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
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| pubmed-article:15350831 | pubmed:meshHeading | pubmed-meshheading:15350831... | lld:pubmed |
| pubmed-article:15350831 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15350831 | pubmed:articleTitle | Nordihydroguaiaretic acid-induced Ca2+ handling and cytotoxicity in human prostate cancer cells. | lld:pubmed |
| pubmed-article:15350831 | pubmed:affiliation | Department of Surgery, Kaohsiung Veterans General Hospital, Taiwan 813. | lld:pubmed |
| pubmed-article:15350831 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15350831 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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