| pubmed-article:15336401 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15336401 | lifeskim:mentions | umls-concept:C0043393 | lld:lifeskim |
| pubmed-article:15336401 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:15336401 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:15336401 | lifeskim:mentions | umls-concept:C0596607 | lld:lifeskim |
| pubmed-article:15336401 | lifeskim:mentions | umls-concept:C0303920 | lld:lifeskim |
| pubmed-article:15336401 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:15336401 | lifeskim:mentions | umls-concept:C0453375 | lld:lifeskim |
| pubmed-article:15336401 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:15336401 | pubmed:dateCreated | 2004-8-31 | lld:pubmed |
| pubmed-article:15336401 | pubmed:abstractText | Monitoring the yeast populations within pickle soaking fluid is imperative for ensuring optimum taste, but these analyses have proven time-consuming and expensive, limiting their industrial application. Here, yeasts were identified in the soaking fluid from Japanese radish pickles using fluorescent PCR amplification of the variable D1/D2 region of the 26S rDNA, followed by analysis with microtemperature-gradient gel electrophoresis (micro-TGGE). This smaller version of the normal TGGE apparatus is capable of analyzing samples 10- to 20-fold faster without sacrificing data quality. Each primer set was labeled with a different fluorescent dye, allowing easy isolation of the various PCR products and identification of the bands corresponding to the various yeasts. The results indicate that fluorescent PCR and micro-TGGE may be a useful new method for rapid, easy monitoring of yeast flora in various food industries. This new method can be used on a daily basis to provide overviews of yeast flora during pickle production, allowing producers to quickly grasp pickle readiness at a single glance. | lld:pubmed |
| pubmed-article:15336401 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15336401 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15336401 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15336401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15336401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15336401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15336401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15336401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15336401 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15336401 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15336401 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:15336401 | pubmed:issn | 0378-1097 | lld:pubmed |
| pubmed-article:15336401 | pubmed:author | pubmed-author:TominagaTatsu... | lld:pubmed |
| pubmed-article:15336401 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15336401 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:15336401 | pubmed:volume | 238 | lld:pubmed |
| pubmed-article:15336401 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15336401 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15336401 | pubmed:pagination | 43-8 | lld:pubmed |
| pubmed-article:15336401 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:meshHeading | pubmed-meshheading:15336401... | lld:pubmed |
| pubmed-article:15336401 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15336401 | pubmed:articleTitle | Rapid identification of pickle yeasts by fluorescent PCR and microtemperature-gradient gel electrophoresis. | lld:pubmed |
| pubmed-article:15336401 | pubmed:affiliation | Saitama Industrial Technology Center North Institute, 2-133, Suehiro, Kumagayashi, Saitama 360-0031, Japan. tominaga@itc-n.pref.saitama.jp | lld:pubmed |
| pubmed-article:15336401 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15336401 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15336401 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15336401 | lld:pubmed |
