| pubmed-article:1531842 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C1383501 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0376525 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0034790 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C1332714 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0205217 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0004083 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0034780 | lld:lifeskim |
| pubmed-article:1531842 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:1531842 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:1531842 | pubmed:dateCreated | 1992-4-9 | lld:pubmed |
| pubmed-article:1531842 | pubmed:abstractText | Exposure of T94, a CD4+ V beta 8-expressing murine Th cell clone, or immediately ex vivo CD4+ T cells to deaggregated, bivalent antibodies specific for either the TCR or CD3 failed to induce an increase in [Ca2+]i, or activation of phosphatidylinositol hydrolysis unless cross-linked with a secondary anti-Ig antibody. In contrast, we show that a combination of two mAb directed against different components of the TCR/CD3 complex (145.2C11, anti-CD3 epsilon and F23.1, anti-V beta 8) successfully induce second messenger formation, that is, without any requirement for a secondary antibody. This requirement for either a secondary antibody or two independent bivalent antibodies to activate second messenger production in T cells suggested that the signal transduction apparatus may be activated by multiple TCR/CD3 complexes being brought together on the T cell surface. This was supported by the observation that conditions inducing increased T cell [Ca2+]i through the TCR/CD3 complex also resulted in aggregation of the TCR/CD3 complex on the T cell surface. Conversely, binding of anti-TCR/CD3 antibodies to the T cell under conditions that did not induce increased [Ca2+]i also failed to induce surface TCR/CD3 redistribution. Cross-linking of the CD4 accessory molecule on T94 also resulted in increased [Ca2+]i, with kinetics similar to those observed after TCR/CD3 oligomerization. CD4 is involved in the recognition of invariant regions of MHC class II during Ag presentation and has been proposed to be associated with TCR/CD3 in the absence of Ag. Aggregation of TCR/CD3 and subsequent second messenger formation was achieved by combinations of mAb to distinct determinants within the complex due to the stable association of these determinants within the T cell membrane. We therefore assessed the functional association of CD4 with the TCR/CD3 complex by examining whether a combination of mAb directed against CD4 and CD3 or TCR induced second messenger formation. We found that anti-CD4 in combination with F23.1 or with 145.2C11 failed to induce increases in [Ca2+]i. Furthermore, mAb to CD4 failed to inhibit the increase in [Ca2+]i observed with the combination of 145.2C11 and F23.1. We therefore conclude that CD4 is not stably associated with TCR or CD3 in the absence of Ag/MHC class II composites. | lld:pubmed |
| pubmed-article:1531842 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1531842 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1531842 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:1531842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1531842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1531842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1531842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1531842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1531842 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1531842 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1531842 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:1531842 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:1531842 | pubmed:author | pubmed-author:JuliusM HMH | lld:pubmed |
| pubmed-article:1531842 | pubmed:author | pubmed-author:RatcliffeM... | lld:pubmed |
| pubmed-article:1531842 | pubmed:author | pubmed-author:CoggeshallK... | lld:pubmed |
| pubmed-article:1531842 | pubmed:author | pubmed-author:NewellM KMK | lld:pubmed |
| pubmed-article:1531842 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1531842 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:1531842 | pubmed:volume | 148 | lld:pubmed |
| pubmed-article:1531842 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1531842 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1531842 | pubmed:pagination | 1643-51 | lld:pubmed |
| pubmed-article:1531842 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:meshHeading | pubmed-meshheading:1531842-... | lld:pubmed |
| pubmed-article:1531842 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1531842 | pubmed:articleTitle | T cell receptor aggregation, but not dimerization, induces increased cytosolic calcium concentrations and reveals a lack of stable association between CD4 and the T cell receptor. | lld:pubmed |
| pubmed-article:1531842 | pubmed:affiliation | Department of Microbiology and Immunology, McGill University, Montreal, Canada. | lld:pubmed |
| pubmed-article:1531842 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1531842 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1531842 | lld:pubmed |
