pubmed-article:15301511 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C0020944 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C1015366 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C1706515 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C1704640 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C0369334 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C0449851 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C0054889 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C0071708 | lld:lifeskim |
pubmed-article:15301511 | lifeskim:mentions | umls-concept:C0331858 | lld:lifeskim |
pubmed-article:15301511 | pubmed:issue | 17 | lld:pubmed |
pubmed-article:15301511 | pubmed:dateCreated | 2004-8-10 | lld:pubmed |
pubmed-article:15301511 | pubmed:abstractText | A novel potentiometric immunosensor for detection of hepatitis B surface antigen (HBsAg) has been developed by means of self-assembly (SA) and opposite-charged adsorption (OCA) techniques to immobilize hepatitis B surface antibody (HBsAb) on a platinum electrode. A cleaned platinum electrode was first pretreated in the presence of 10% HNO3 and 2.5% K2CrO4 solution and held at -1.5 V (vs SCE) for 1 min to make it negatively charged and then immersed in a mixing solution containing hepatitis B surface antibody, colloidal gold (Au), and polyvinyl butyral (PVB). Finally, HBsAb was successfully immobilized onto the surface of the negatively charged platinum electrode modified nanosized gold and PVB sol-gel matrixes. The modified procedure was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The immobilized hepatitis B surface antibody exhibited direct electrochemical behavior toward hepatitis B surface antigen (HBsAg). The performance and factors influencing the performance of the resulting immunosensor were studied in detail. More than 95.7% of the results of the human serum samples obtained by this method were in agreement with those obtained by enzyme-linked immunosorbent assays (ELISAs). The resulting immunosensor exhibited fast potentiometric response (<3 min) to HBsAg. The detection limit of the immunosensor was 2.3 ng.mL(-1), and the linear range was from 8 to 1280 ng.mL(-1). Moreover, the studied immunosensor exhibited high sensitivity, good reproducibility, and long-term stability (>6 months). | lld:pubmed |
pubmed-article:15301511 | pubmed:language | eng | lld:pubmed |
pubmed-article:15301511 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:15301511 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:15301511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:15301511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:15301511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:15301511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:15301511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:15301511 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:15301511 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:15301511 | pubmed:month | Aug | lld:pubmed |
pubmed-article:15301511 | pubmed:issn | 0743-7463 | lld:pubmed |
pubmed-article:15301511 | pubmed:author | pubmed-author:LiuYanY | lld:pubmed |
pubmed-article:15301511 | pubmed:author | pubmed-author:ZhongXiaX | lld:pubmed |
pubmed-article:15301511 | pubmed:author | pubmed-author:YuanRuoR | lld:pubmed |
pubmed-article:15301511 | pubmed:author | pubmed-author:TangDianpingD | lld:pubmed |
pubmed-article:15301511 | pubmed:author | pubmed-author:ChaiYaqinY | lld:pubmed |
pubmed-article:15301511 | pubmed:author | pubmed-author:DaiJianyuanJ | lld:pubmed |
pubmed-article:15301511 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:15301511 | pubmed:day | 17 | lld:pubmed |
pubmed-article:15301511 | pubmed:volume | 20 | lld:pubmed |
pubmed-article:15301511 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:15301511 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:15301511 | pubmed:pagination | 7240-5 | lld:pubmed |
pubmed-article:15301511 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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pubmed-article:15301511 | pubmed:year | 2004 | lld:pubmed |
pubmed-article:15301511 | pubmed:articleTitle | Ultrasensitive potentiometric immunosensor based on SA and OCA techniques for immobilization of HBsAb with colloidal Au and polyvinyl butyral as matrixes. | lld:pubmed |
pubmed-article:15301511 | pubmed:affiliation | Chong Qing Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest China Normal University, Chongqing 400715, China. yuanruo@swnu.edu.cn | lld:pubmed |
pubmed-article:15301511 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:15301511 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |