| pubmed-article:15292658 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15292658 | lifeskim:mentions | umls-concept:C0221908 | lld:lifeskim |
| pubmed-article:15292658 | lifeskim:mentions | umls-concept:C0024432 | lld:lifeskim |
| pubmed-article:15292658 | lifeskim:mentions | umls-concept:C0025519 | lld:lifeskim |
| pubmed-article:15292658 | lifeskim:mentions | umls-concept:C0031308 | lld:lifeskim |
| pubmed-article:15292658 | lifeskim:mentions | umls-concept:C0035323 | lld:lifeskim |
| pubmed-article:15292658 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15292658 | pubmed:dateCreated | 2004-8-4 | lld:pubmed |
| pubmed-article:15292658 | pubmed:abstractText | The purpose of this study is to compare primary human retinal pigment epithelium (RPE) cells with respect to particle uptake and further processing steps with immunological phagocytes for a better understanding of the possible role of RPE cells in triggering autoimmune diseases in the eye. We investigated the similarities of human RPE and monocytes/macrophages studying the uptake of fluorescein- and europium-labeled synthetic microparticles and microbial pathogens by human and bovine RPE cultures and a permanent RPE cell line (CRL). The uptake was monitored by laser scanning microscopy, flow cytometry and time-resolved fluorescence analysis; for comparison, macrophages and a macrophage-like cell line (MonoMac6) were used. A size-dependent uptake was seen in primary RPE cultures as well as in CRL, showing a preferential uptake of smaller beads followed by Staphylococcus aureus and Escherichia coli. Opsonization with serum caused a modest increase in bacteria uptake, but in contrast to macrophages, the classical complement receptors were not found on RPE cells. Living bacteria were also ingested in a time-dependent manner, but, as no intracellular overgrowth was observed, we further investigated the oxidative ability of RPE as a possible mechanism for microbial suppression. Unlike macrophages/granulocytes, no respiratory burst was detected in RPE cells, but, comparable to MonoMac6, IFN-gamma induced neopterin in the human RPE. Interestingly a diurnal rhythm of phagocytosis was observed which was influenced by light exposure suggesting that RPE cells maintain their circadian rhythm also in cell culture to a certain extent. This study further demonstrates that in addition to similar phagocytic properties the RPE still shows substantial metabolic differences in comparison to blood-derived phagocytes. | lld:pubmed |
| pubmed-article:15292658 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15292658 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15292658 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15292658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15292658 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15292658 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15292658 | pubmed:issn | 0030-3747 | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:NussbaumerWal... | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:FuchsDietmarD | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:BöckGüntherG | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:HuemerHartwig... | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:GöttingerWolf... | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:SgoncRoswitha... | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:IrschickEveli... | lld:pubmed |
| pubmed-article:15292658 | pubmed:author | pubmed-author:WolfHugoH | lld:pubmed |
| pubmed-article:15292658 | pubmed:copyrightInfo | Copyright 2004 S. Karger AG, Basel | lld:pubmed |
| pubmed-article:15292658 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15292658 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:15292658 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15292658 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15292658 | pubmed:pagination | 200-10 | lld:pubmed |
| pubmed-article:15292658 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:meshHeading | pubmed-meshheading:15292658... | lld:pubmed |
| pubmed-article:15292658 | pubmed:articleTitle | Retinal pigment epithelial phagocytosis and metabolism differ from those of macrophages. | lld:pubmed |
| pubmed-article:15292658 | pubmed:affiliation | Department of Ophthalmology, University Hospital Innsbruck, Innsbruck, Austria. eveline.irschick@uklibk.ac.at | lld:pubmed |
| pubmed-article:15292658 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15292658 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15292658 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15292658 | lld:pubmed |
