| pubmed-article:15277519 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15277519 | lifeskim:mentions | umls-concept:C2717940 | lld:lifeskim |
| pubmed-article:15277519 | lifeskim:mentions | umls-concept:C1157326 | lld:lifeskim |
| pubmed-article:15277519 | lifeskim:mentions | umls-concept:C0428631 | lld:lifeskim |
| pubmed-article:15277519 | lifeskim:mentions | umls-concept:C0242414 | lld:lifeskim |
| pubmed-article:15277519 | pubmed:issue | 40 | lld:pubmed |
| pubmed-article:15277519 | pubmed:dateCreated | 2004-9-27 | lld:pubmed |
| pubmed-article:15277519 | pubmed:abstractText | A major product of mitochondrial and peroxisomal beta-oxidation is acetyl-CoA, which is essential for multiple cellular processes. The relative role of peroxisomal beta-oxidation of long chain fatty acids and the fate of its oxidation products are poorly understood and are the subjects of our research. In this report we describe a study of beta-oxidation of palmitate and stearate using HepG2 cells cultured in the presence of multiple concentrations of [U-(13)C(18)]stearate or [U-(13)C(16)] palmitate. Using mass isotopomer analysis we determined the enrichments of acetyl-CoA used in de novo lipogenesis (cytosolic pool), in the tricarboxylic acid cycle (glutamate pool), and in chain elongation of stearate (peroxisomal pool). Cells treated with 0.1 mm [U-(13)C(18)]stearate had markedly disparate acetyl-CoA enrichments (1.1% cytosolic, 1.1% glutamate, 10.7% peroxisomal) with increased absolute levels of C20:0, C22:0, and C24:0. However, cells treated with 0.1 mm [U-(13)C(16)]palmitate had a lower peroxisomal enrichment (1.8% cytosolic, 1.6% glutamate, and 1.1% peroxisomal). At higher fatty acid concentrations, acetyl-CoA enrichments in these compartments were proportionally increased. Chain shortening and elongation was determined using spectral analysis. Chain shortening of stearate in peroxisomes generates acetyl-CoA, which is subsequently used in the chain elongation of a second stearate molecule to form very long chain fatty acids. Chain elongation of palmitate to stearate appeared to occur in a different compartment. Our results suggest that 1) chain elongation activity is a useful and novel probe for peroxisomal beta-oxidation and 2) chain shortening contributes a substantial fraction of the acetyl-CoA used for fatty acid elongation in HepG2 cells. | lld:pubmed |
| pubmed-article:15277519 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15277519 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15277519 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15277519 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15277519 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:15277519 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:15277519 | pubmed:author | pubmed-author:SunN NNN | lld:pubmed |
| pubmed-article:15277519 | pubmed:author | pubmed-author:BassilianSara... | lld:pubmed |
| pubmed-article:15277519 | pubmed:author | pubmed-author:Paul... | lld:pubmed |
| pubmed-article:15277519 | pubmed:author | pubmed-author:WongDerek ADA | lld:pubmed |
| pubmed-article:15277519 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15277519 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:15277519 | pubmed:volume | 279 | lld:pubmed |
| pubmed-article:15277519 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15277519 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15277519 | pubmed:pagination | 41302-9 | lld:pubmed |
| pubmed-article:15277519 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:meshHeading | pubmed-meshheading:15277519... | lld:pubmed |
| pubmed-article:15277519 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15277519 | pubmed:articleTitle | Coordination of peroxisomal beta-oxidation and fatty acid elongation in HepG2 cells. | lld:pubmed |
| pubmed-article:15277519 | pubmed:affiliation | Department of Pediatrics, Harbor-UCLA Research and Education Institute, UCLA School of Medicine, Torrance, California 90502, USA. | lld:pubmed |
| pubmed-article:15277519 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15277519 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15277519 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15277519 | lld:pubmed |
