| pubmed-article:15270959 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C0008059 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C0442034 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C1704632 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C0031139 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C0871261 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C1423842 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C2911692 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C1706817 | lld:lifeskim |
| pubmed-article:15270959 | lifeskim:mentions | umls-concept:C0205191 | lld:lifeskim |
| pubmed-article:15270959 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:15270959 | pubmed:dateCreated | 2004-7-23 | lld:pubmed |
| pubmed-article:15270959 | pubmed:abstractText | Peritoneal T cell responses can be polarized toward Th1 or Th2 in children on chronic peritoneal dialysis. Previous studies on the peritoneal immune system described the presence of activated T lymphocytes in peritoneal effluents from subjects on chronic peritoneal dialysis (CPD). Since Th1/Th2 polarized response can influence the outcome of specific infectious diseases, we investigated if activated Th1/Th2 cells can be detected in peritoneal effluents during peritoneal dialysis, in order to better understand the role of T cells in the mechanisms of peritoneal defense. We have studied 8 children (4 males, 4 females, mean age 5.8 +/- 5.7 years, range 0.3-13.4) on CPD. Peritoneal cells have been isolated from peritoneal effluents by centrifugation. Immunofluorescent staining of intracellular cytokines for flow cytometric analysis was used to detect the percentage of T cells producing either IFN-gamma (Th1) or IL-4 (Th2). In the initial study 3 months after CPD initiation, high percentages of IFN-gamma positive peritoneal T cells (38% and 63%) were detected in two subjects; this finding is consistent with a Th1 polarization of peritoneal T cells. In another subject, high percentages of IL-4 positive T cells (31%) were detected, suggesting a Th2 polarization of peritoneal T cell response. Small amounts of either Th1 or Th2 T cells (2-4%) were also detected in the other subjects. At the 1 year follow-up, Th1 polarization persisted in one subject (18% IFN-gamma positive peritoneal T cells), in another a shift from Th1 to Th2 was observed, and in the other subject a down regulation of both T cell subsets occurred. The finding that a predominance of T cells producing either IFN-gamma or IL-4 was found in 3 out of 8 children strongly suggests that peritoneal T cell responses can be polarized toward Th1 or Th2. The decrease of Th1 and/or Th2 polarized T cells in the peritoneum of 4 out of 6 subjects (after 1 year) suggests that CPD can play an immunosuppressive role on T cell peritoneal responses. Further studies are needed in order to define whether different T helper activation patterns are associated with a higher risk of peritoneal infection or of peritoneal damage. | lld:pubmed |
| pubmed-article:15270959 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15270959 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15270959 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15270959 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15270959 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15270959 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:15270959 | pubmed:issn | 0160-564X | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:PistoiaVitoV | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:VerrinaEnrico... | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:PerfumoFrance... | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:PrigioneIgnaz... | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:ChiesaSabrina... | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:BarbanoGianca... | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:VigoGiacomoG | lld:pubmed |
| pubmed-article:15270959 | pubmed:author | pubmed-author:CappaFrancesc... | lld:pubmed |
| pubmed-article:15270959 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15270959 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:15270959 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15270959 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15270959 | pubmed:pagination | 750-2 | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:meshHeading | pubmed-meshheading:15270959... | lld:pubmed |
| pubmed-article:15270959 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15270959 | pubmed:articleTitle | Peritoneal T cell responses can be polarized toward Th1 or Th2 in children on chronic peritoneal dialysis. | lld:pubmed |
| pubmed-article:15270959 | pubmed:affiliation | Nephrology Department, G. Gaslini Children's Hospital, Genoa, Italy. | lld:pubmed |
| pubmed-article:15270959 | pubmed:publicationType | Journal Article | lld:pubmed |
