| pubmed-article:1527089 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1527089 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:1527089 | lifeskim:mentions | umls-concept:C0021760 | lld:lifeskim |
| pubmed-article:1527089 | lifeskim:mentions | umls-concept:C0005767 | lld:lifeskim |
| pubmed-article:1527089 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:1527089 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:1527089 | pubmed:issue | 27 | lld:pubmed |
| pubmed-article:1527089 | pubmed:dateCreated | 1992-10-22 | lld:pubmed |
| pubmed-article:1527089 | pubmed:abstractText | The biochemical nature of endogenous interleukin-6 (IL-6) as it exists in human serum or plasma was investigated. Serum from a patient following bone marrow (BM) transplantation and fresh plasma samples from patients with epidermolysis bullosa or psoriasis, as well as from normal volunteers, were fractionated through G-200 columns and each of the eluted fractions assayed for IL (interleukin)-6 content using enzyme-linked immunosorbent assays (ELISAs) based on the monoclonal antibody (mAb) pairs IG61/5IL6 or 4IL6/5IL6 and in the B9 hybridoma growth factor bioassay. The IG61/5IL6 ELISA and the B9 assay detected IL-6 in BM serum almost exclusively of molecular mass approximately 20 kDa. In contrast, the 4IL6/5IL6 ELISA detected strong IL-6 immunoreactivity in complexes of size 100-150 and 400-500 kDa. IL-6 present in the 100-150- and 400-500-kDa complexes was purified by immunoaffinity chromatography through a 5IL6 mAb column. The 5IL6 mAb immunoaffinity column eluate of the respective pools from BM serum contained IL-6 at concentrations approaching 1 microgram/ml as characterized by Western blotting. Sufficient IL-6 and associated proteins were purified by 5IL6 mAb immunoaffinity column chromatography of the 100-150-kDa complex from 0.8 ml of BM serum to allow (i) verification of three of the polypeptides as IL-6 by amino-terminal sequencing (estimate of IL-6 in original serum sample: 5-10 micrograms/ml), (ii) identification by amino acid sequencing of the "associated" proteins as complement factor C3b (carboxyl-terminal of the alpha-chain), complement factor C4b (gamma-chain), C-reactive protein, and albumin, and (iii) detection of an "associated" polypeptide consistent with the soluble IL-6 receptor. Taken together, these data establish that IL-6 is present at unexpectedly high concentrations in human blood in novel biochemical complexes that include other plasma proteins, which in turn, can camouflage IL-6 immunoreactivity and bioactivity as measured in conventional assays. | lld:pubmed |
| pubmed-article:1527089 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1527089 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1527089 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1527089 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1527089 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1527089 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1527089 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1527089 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1527089 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1527089 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1527089 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:1527089 | pubmed:author | pubmed-author:AllisonA CAC | lld:pubmed |
| pubmed-article:1527089 | pubmed:author | pubmed-author:SehgalP BPB | lld:pubmed |
| pubmed-article:1527089 | pubmed:author | pubmed-author:KennelJ HJH | lld:pubmed |
| pubmed-article:1527089 | pubmed:author | pubmed-author:LeuM LML | lld:pubmed |
| pubmed-article:1527089 | pubmed:author | pubmed-author:IdaNN | lld:pubmed |
| pubmed-article:1527089 | pubmed:author | pubmed-author:ViguetHH | lld:pubmed |
| pubmed-article:1527089 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1527089 | pubmed:day | 25 | lld:pubmed |
| pubmed-article:1527089 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:1527089 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1527089 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1527089 | pubmed:pagination | 19698-704 | lld:pubmed |
| pubmed-article:1527089 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
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| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
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| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
| pubmed-article:1527089 | pubmed:meshHeading | pubmed-meshheading:1527089-... | lld:pubmed |
| pubmed-article:1527089 | pubmed:year | 1992 | lld:pubmed |
| pubmed-article:1527089 | pubmed:articleTitle | High levels of "complexed" interleukin-6 in human blood. | lld:pubmed |
| pubmed-article:1527089 | pubmed:affiliation | Department of Microbiology and Immunology, New York Medical College, Valhalla 10595. | lld:pubmed |
| pubmed-article:1527089 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1527089 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:1527089 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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