| pubmed-article:15269532 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15269532 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:15269532 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:15269532 | lifeskim:mentions | umls-concept:C0383327 | lld:lifeskim |
| pubmed-article:15269532 | lifeskim:mentions | umls-concept:C0037119 | lld:lifeskim |
| pubmed-article:15269532 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:15269532 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:15269532 | pubmed:dateCreated | 2004-7-22 | lld:pubmed |
| pubmed-article:15269532 | pubmed:abstractText | Recombinant porcine mature interleukin-18 (rPomIL-18) has been purified from the haemolymph of silkworms. After co-infection of two recombinant baculoviruses (BmAcpVL1392-IL-18-His and BmAcpVL1392-casp-1) into the silkworm, rPomIL-18 was produced and secreted into the haemolymph. Polyethylene glycol (PEG) 6000 was added to the haemolymph at 8% (v/w) to precipitate storage proteins which would otherwise bind non-specifically to the metal chelating column and the supernatant then was applied to Sepharose bonded with Ni2+. rPomIL-18 was eluted from the column using 100 mM imidazole buffer at pH 8 with a purity of 93.6%. Approximately 5.3 mg purified rPomIL-18 was obtained from 22 ml haemolymph. It could induce interferon-gamma formation from porcine peripheral blood mononuclear cells. | lld:pubmed |
| pubmed-article:15269532 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15269532 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15269532 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15269532 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15269532 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15269532 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15269532 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15269532 | pubmed:issn | 0141-5492 | lld:pubmed |
| pubmed-article:15269532 | pubmed:author | pubmed-author:MunetaYoshihi... | lld:pubmed |
| pubmed-article:15269532 | pubmed:author | pubmed-author:MoriYasuyukiY | lld:pubmed |
| pubmed-article:15269532 | pubmed:author | pubmed-author:YokomizoYuich... | lld:pubmed |
| pubmed-article:15269532 | pubmed:author | pubmed-author:NagayaHidekaz... | lld:pubmed |
| pubmed-article:15269532 | pubmed:author | pubmed-author:EnomotoChiaki... | lld:pubmed |
| pubmed-article:15269532 | pubmed:author | pubmed-author:MatsumotoSaya... | lld:pubmed |
| pubmed-article:15269532 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15269532 | pubmed:volume | 26 | lld:pubmed |
| pubmed-article:15269532 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15269532 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15269532 | pubmed:pagination | 869-73 | lld:pubmed |
| pubmed-article:15269532 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:meshHeading | pubmed-meshheading:15269532... | lld:pubmed |
| pubmed-article:15269532 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15269532 | pubmed:articleTitle | Method for purifying porcine mature interleukin-18 from silkworm haemolymph. | lld:pubmed |
| pubmed-article:15269532 | pubmed:affiliation | Katakura Industries Co., Ltd., 1548 Shimo-okutomi, Sayama, Saitama 350-1332, Japan. h.nagaya@katakura.co.jp | lld:pubmed |
| pubmed-article:15269532 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15269532 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15269532 | lld:pubmed |
