| pubmed-article:15253151 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C0242610 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C0036576 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C1412111 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C1519613 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:15253151 | lifeskim:mentions | umls-concept:C1542147 | lld:lifeskim |
| pubmed-article:15253151 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:15253151 | pubmed:dateCreated | 2004-7-15 | lld:pubmed |
| pubmed-article:15253151 | pubmed:abstractText | Human ACAT 1 cDNA K1 was first cloned and functionally expressed in 1993. There are two adjacent in-frame AUG codons, AUG(1397-1399) and AUG(1415-1417), at 5'-terminus of the open reading frame (ORF, nt 1397-3049) of human ACAT1 mRNA corresponding to cDNA K1. In current work, these two adjacent inframe AUGs at 5'-terminus of the predicted ORF (5'-ORF-AUGs) as start codons for translation initiation of human ACAT1 mRNA were characterized in detail. Codon mutations indicated that both of these two adjacent 5'-ORF-AUGs can be selected as start codons but the first 5'-ORF-AUG(1397-1399) is a main start codon consistent with that of the predicted ORF of human ACAT1 mRNA. Further deletion and mutation analyses demonstrated that a stable upstream stem-loop structure enhanced the selection of the first 5'-ORF-AUG(1397 -1399) as a main start codon, in addition to upstream nucleotide A in the -3 position, which is a key site of Kozak sequence. In addition, result of ACAT1 enzymatic activity assay showed no obvious difference between these two ACAT1 proteins respectively initiated from the two adjacent 5'-ORF-AUGs. This work showed that a stable upstream stem-loop structure could modulate the start codon selection during translation initiation of mRNAs that contain adjacent multi-5'-ORF-AUGs. | lld:pubmed |
| pubmed-article:15253151 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15253151 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15253151 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15253151 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15253151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15253151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15253151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15253151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15253151 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15253151 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15253151 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15253151 | pubmed:issn | 1672-9145 | lld:pubmed |
| pubmed-article:15253151 | pubmed:author | pubmed-author:ChangCatherin... | lld:pubmed |
| pubmed-article:15253151 | pubmed:author | pubmed-author:ChangTa-YuanT... | lld:pubmed |
| pubmed-article:15253151 | pubmed:author | pubmed-author:YangLiL | lld:pubmed |
| pubmed-article:15253151 | pubmed:author | pubmed-author:LiBo-LiangBL | lld:pubmed |
| pubmed-article:15253151 | pubmed:author | pubmed-author:JiangChenC | lld:pubmed |
| pubmed-article:15253151 | pubmed:author | pubmed-author:YangXin-YingX... | lld:pubmed |
| pubmed-article:15253151 | pubmed:author | pubmed-author:WangZhen-Zhen... | lld:pubmed |
| pubmed-article:15253151 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15253151 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:15253151 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15253151 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15253151 | pubmed:pagination | 259-68 | lld:pubmed |
| pubmed-article:15253151 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
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| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:meshHeading | pubmed-meshheading:15253151... | lld:pubmed |
| pubmed-article:15253151 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15253151 | pubmed:articleTitle | A stable upstream stem-loop structure enhances selection of the first 5'-ORF-AUG as a main start codon for translation initiation of human ACAT1 mRNA. | lld:pubmed |
| pubmed-article:15253151 | pubmed:affiliation | State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences. | lld:pubmed |
| pubmed-article:15253151 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15253151 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15253151 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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