| pubmed-article:15231839 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C0001455 | lld:lifeskim |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C1524075 | lld:lifeskim |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C0183210 | lld:lifeskim |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:15231839 | lifeskim:mentions | umls-concept:C0337112 | lld:lifeskim |
| pubmed-article:15231839 | pubmed:issue | 36 | lld:pubmed |
| pubmed-article:15231839 | pubmed:dateCreated | 2004-8-30 | lld:pubmed |
| pubmed-article:15231839 | pubmed:abstractText | cAMP is a universal second messenger of many G-protein-coupled receptors and regulates a wide variety of cellular events. cAMP exerts its effects via cAMP-dependent protein kinase (PKA), cAMP-gated ion channels, and two isoforms of exchange protein directly activated by cAMP (Epac). Here we report the development of novel fluorescent indicators for cAMP based on the cAMP-binding domains of Epac and PKA. Fluorescence resonance energy transfer between variants of green fluorescent protein (enhanced cyan fluorescent protein and enhanced yellow fluorescent protein) fused directly to the cAMP-binding domains was used to analyze spatial and temporal aspects of cAMP-signaling in different cells. In contrast to previously developed PKA-based indicators, these probes are comprised of only a single binding site lacking cooperativity, catalytic properties, and interactions with other proteins and thereby allow us to easily image free intracellular cAMP and rapid signaling events. Rapid beta-adrenergic receptor-induced cAMP signals were observed to travel with high speed ( approximately 40 microm/s) throughout the entire cell body of hippocampal neurons and peritoneal macrophages. The developed indicators could be ubiquitously applied to studying cAMP, its physiological role and spatio-temporal regulation. | lld:pubmed |
| pubmed-article:15231839 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15231839 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231839 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15231839 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15231839 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15231839 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:15231839 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:15231839 | pubmed:author | pubmed-author:LohseMartin... | lld:pubmed |
| pubmed-article:15231839 | pubmed:author | pubmed-author:HeinLutzL | lld:pubmed |
| pubmed-article:15231839 | pubmed:author | pubmed-author:BünemannMorit... | lld:pubmed |
| pubmed-article:15231839 | pubmed:author | pubmed-author:HannawackerAn... | lld:pubmed |
| pubmed-article:15231839 | pubmed:author | pubmed-author:NikolaevViach... | lld:pubmed |
| pubmed-article:15231839 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15231839 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:15231839 | pubmed:volume | 279 | lld:pubmed |
| pubmed-article:15231839 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15231839 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15231839 | pubmed:pagination | 37215-8 | lld:pubmed |
| pubmed-article:15231839 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:meshHeading | pubmed-meshheading:15231839... | lld:pubmed |
| pubmed-article:15231839 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15231839 | pubmed:articleTitle | Novel single chain cAMP sensors for receptor-induced signal propagation. | lld:pubmed |
| pubmed-article:15231839 | pubmed:affiliation | Institute of Pharmacology and Toxicology, University of Würzburg, Versbacherstrasse 9, D-97078 Würzburg, Germany. | lld:pubmed |
| pubmed-article:15231839 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15231839 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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