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pubmed-article:15215587pubmed:dateCreated2004-6-24lld:pubmed
pubmed-article:15215587pubmed:abstractTextChanges in the levels of thiamin-binding globulin and thiamin in wheat seeds during maturation and germination were studied. The thiamin-binding activity of the seed proteins increased with seed development after flowering. The thiamin content of the seeds also increased with development. Thiamin-binding activity decreased during seed germination. On the other hand, immunological analysis using an antibody directed against the thiamin-binding protein isolated from wheat seeds showed that the thiamin-binding globulin accumulated in the aleurone layer of the seeds during maturation, and then the protein was degraded and disappeared during seed germination. These results suggested that the thiamin-binding globulin of wheat seeds was synthesized and accumulated in the aleurone layer of the seeds with seed development, similar to the thiamin-binding albumin in sesame seeds, and that thiamin bound to the thiamin-binding globulin in the dormant wheat seeds for germ growth during germination.lld:pubmed
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pubmed-article:15215587pubmed:issn0916-8451lld:pubmed
pubmed-article:15215587pubmed:authorpubmed-author:KawamuraYukio...lld:pubmed
pubmed-article:15215587pubmed:authorpubmed-author:WatanabeKatsu...lld:pubmed
pubmed-article:15215587pubmed:authorpubmed-author:AdachiTakashi...lld:pubmed
pubmed-article:15215587pubmed:authorpubmed-author:NishidaNaokoNlld:pubmed
pubmed-article:15215587pubmed:authorpubmed-author:UedaMotokoMlld:pubmed
pubmed-article:15215587pubmed:authorpubmed-author:MitsunagaTosh...lld:pubmed
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pubmed-article:15215587pubmed:volume68lld:pubmed
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pubmed-article:15215587pubmed:pagination1243-8lld:pubmed
pubmed-article:15215587pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:15215587pubmed:year2004lld:pubmed
pubmed-article:15215587pubmed:articleTitleAccumulation and degradation of thiamin-binding protein and level of thiamin in wheat seeds during seed maturation and germination.lld:pubmed
pubmed-article:15215587pubmed:affiliationDepartment of Food and Nutrition, Faculty of Agriculture, Kinki University, Nakamachi, Nara, Japan. kwatanabe@nara.kindai.ac.jplld:pubmed
pubmed-article:15215587pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:15215587pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed