Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:1521542rdf:typepubmed:Citationlld:pubmed
pubmed-article:1521542lifeskim:mentionsumls-concept:C0014442lld:lifeskim
pubmed-article:1521542lifeskim:mentionsumls-concept:C1383501lld:lifeskim
pubmed-article:1521542lifeskim:mentionsumls-concept:C0679622lld:lifeskim
pubmed-article:1521542lifeskim:mentionsumls-concept:C0205314lld:lifeskim
pubmed-article:1521542lifeskim:mentionsumls-concept:C0086149lld:lifeskim
pubmed-article:1521542pubmed:issue2lld:pubmed
pubmed-article:1521542pubmed:dateCreated1992-10-13lld:pubmed
pubmed-article:1521542pubmed:abstractTextDifferentiation-inducing factor 1 (DIF-1) is a dichlorinated alkyl phenone (1-[(3,5-dichloro-2,6-dihydroxy-4-methoxy)phenyl]hexan-1-one) from Dictyostelium discoideum, that induces amoebae to differentiate into stalk cells. It was shown previously that DIF-1 is rapidly metabolized into a series of more polar compounds by living cells [Traynor, D. & Kay, R.R. (1991) J. Biol. Chem. 266, 5291-5297]. The first step in DIF-1 metabolism is the formation of DIF metabolite 1 (now known to be DIF-3) by a monodechlorination. We report here the discovery of the enzyme activity catalyzing this dechlorination. A very sensitive enzyme assay was developed, using [3H]DIF-1 and a TLC system to separate DIF-1 from the product, DIF-3. DIF-1 3(5)-dechlorinase is present in the high-speed supernatant of cell lysates, and uses glutathione, at physiological concentrations, as cofactor. Kinetic measurements indicate a Km for DIF-1 of about 70 nM. The enzyme activity is inhibited by DIF-2 (the pentan-1-one analogue of DIF-1), with a median inhibitor concentration (IC50) of 1 microM, and DIF-3 (IC50 = 5 microM), which presumably act as substrates, but other compounds structurally related to DIF-1 were much less effective. Aurothioglucose, an inhibitor of selenocysteine enzymes, inhibited DIF-1 3(5)-dechlorinase with IC50 = 100 nM. DIF-1 3(5)-dechlorinase activity is developmentally regulated. It is essentially absent from growing cells and increases at the end of aggregation to reach a first peak of activity at the first finger stage, with a further rise at culmination.lld:pubmed
pubmed-article:1521542pubmed:languageenglld:pubmed
pubmed-article:1521542pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1521542pubmed:citationSubsetIMlld:pubmed
pubmed-article:1521542pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1521542pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1521542pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1521542pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1521542pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1521542pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:1521542pubmed:statusMEDLINElld:pubmed
pubmed-article:1521542pubmed:monthSeplld:pubmed
pubmed-article:1521542pubmed:issn0014-2956lld:pubmed
pubmed-article:1521542pubmed:authorpubmed-author:LINS CSClld:pubmed
pubmed-article:1521542pubmed:authorpubmed-author:InsallRRlld:pubmed
pubmed-article:1521542pubmed:authorpubmed-author:NaylerOOlld:pubmed
pubmed-article:1521542pubmed:issnTypePrintlld:pubmed
pubmed-article:1521542pubmed:day1lld:pubmed
pubmed-article:1521542pubmed:volume208lld:pubmed
pubmed-article:1521542pubmed:ownerNLMlld:pubmed
pubmed-article:1521542pubmed:authorsCompleteYlld:pubmed
pubmed-article:1521542pubmed:pagination531-6lld:pubmed
pubmed-article:1521542pubmed:dateRevised2007-7-23lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:meshHeadingpubmed-meshheading:1521542-...lld:pubmed
pubmed-article:1521542pubmed:year1992lld:pubmed
pubmed-article:1521542pubmed:articleTitleDifferentiation-inducing-factor dechlorinase, a novel cytosolic dechlorinating enzyme from Dictyostelium discoideum.lld:pubmed
pubmed-article:1521542pubmed:affiliationMRC Laboratory of Molecular Biology, Cambridge, England.lld:pubmed
pubmed-article:1521542pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1521542pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1521542lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1521542lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:1521542lld:pubmed