| pubmed-article:15196917 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15196917 | lifeskim:mentions | umls-concept:C0596402 | lld:lifeskim |
| pubmed-article:15196917 | lifeskim:mentions | umls-concept:C0035542 | lld:lifeskim |
| pubmed-article:15196917 | lifeskim:mentions | umls-concept:C0380603 | lld:lifeskim |
| pubmed-article:15196917 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:15196917 | lifeskim:mentions | umls-concept:C1999216 | lld:lifeskim |
| pubmed-article:15196917 | lifeskim:mentions | umls-concept:C0332206 | lld:lifeskim |
| pubmed-article:15196917 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:15196917 | pubmed:issue | 1-3 | lld:pubmed |
| pubmed-article:15196917 | pubmed:dateCreated | 2004-6-15 | lld:pubmed |
| pubmed-article:15196917 | pubmed:abstractText | Basic fibroblast growth factor (bFGF) was inserted in the middle of human ribonuclease 1 (RNase1) sequence at an RNase inhibitor (RI)-binding site (Gly89) by a new gene fusion technique, insertional-fusion. The resultant insertional-fusion protein (CL-RFN89) was active both as bFGF and as RNase. Furthermore, it acquired an additional ability of evading RI through steric blockade of RI-binding caused by fused bFGF domain. As a result, CL-RFN89 showed stronger growth inhibition on B16/BL6 melanoma cells than an RI-sensitive tandem fusion protein. Thus, the insertional-fusion technique increases accessible positions for gene fusion on RNase, resulting in construction of a potent cytotoxic RNase. | lld:pubmed |
| pubmed-article:15196917 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15196917 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15196917 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15196917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15196917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15196917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15196917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15196917 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15196917 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15196917 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15196917 | pubmed:issn | 0014-5793 | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:TadaHirokoH | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:YamadaHidenor... | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:SenoMasaharuM | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:FutamiJunichi... | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:KosakaMegumiM | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:OnizukaMasayu... | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:MurakiKazukoK | lld:pubmed |
| pubmed-article:15196917 | pubmed:author | pubmed-author:MasuzawaWatar... | lld:pubmed |
| pubmed-article:15196917 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15196917 | pubmed:day | 18 | lld:pubmed |
| pubmed-article:15196917 | pubmed:volume | 568 | lld:pubmed |
| pubmed-article:15196917 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15196917 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15196917 | pubmed:pagination | 39-43 | lld:pubmed |
| pubmed-article:15196917 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:meshHeading | pubmed-meshheading:15196917... | lld:pubmed |
| pubmed-article:15196917 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15196917 | pubmed:articleTitle | Insertional-fusion of basic fibroblast growth factor endowed ribonuclease 1 with enhanced cytotoxicity by steric blockade of inhibitor interaction. | lld:pubmed |
| pubmed-article:15196917 | pubmed:affiliation | Department of Bioscience and Biotechnology, Faculty of Engineering, Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan. tadahrk@biotech.okayama-u.ac.jp | lld:pubmed |
| pubmed-article:15196917 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15196917 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15196917 | lld:pubmed |
