| pubmed-article:15193389 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15193389 | lifeskim:mentions | umls-concept:C0019704 | lld:lifeskim |
| pubmed-article:15193389 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:15193389 | lifeskim:mentions | umls-concept:C0301872 | lld:lifeskim |
| pubmed-article:15193389 | lifeskim:mentions | umls-concept:C1450054 | lld:lifeskim |
| pubmed-article:15193389 | lifeskim:mentions | umls-concept:C0442821 | lld:lifeskim |
| pubmed-article:15193389 | lifeskim:mentions | umls-concept:C0961954 | lld:lifeskim |
| pubmed-article:15193389 | lifeskim:mentions | umls-concept:C1100939 | lld:lifeskim |
| pubmed-article:15193389 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:15193389 | pubmed:dateCreated | 2004-6-14 | lld:pubmed |
| pubmed-article:15193389 | pubmed:abstractText | A significant emphasis has been placed on the development of adjuvants and/or delivery systems to improve both antibody production and cell-mediated immune responses. We previously reported on a novel anionic nanoparticle, which led to enhanced humoral and T helper type-1 (Th1) biased immune responses in mice when coated with cationized model antigen. Tat (1-72) is a conserved regulatory HIV-1 protein. It was hypothesized that HIV vaccine strategies employing Tat (1-72) may be a promising approach. Although previous reports have suggested that Tat (1-86) may be immunosuppressive, it was demonstrated in this present study that Tat (1-72) was not immunosuppressive when co-administered to mice with ovalbumin (OVA). Tat (1-72) was coated on novel anionic nanoparticles. BALB/c mice were immunized with Tat (5 microg)-coated nanoparticles (15 microg) by subcutaneous injection on days 0 and 14. Antibody and cytokine release were determined on day 28 and compared to Tat (5 microg) adjuvanted with Alum (15 microg) as a Th2 control, Tat (5 microg) adjuvanted with Lipid A (50 microg) as a Th1 control. Immunization of BALB/c mice with Tat-coated nanoparticles resulted in antibody levels (IgG and IgM) comparable to those elicited from Tat and Alum. However, Tat-coated nanoparticles led to a Th1 biased immune response. The IFN-gamma release from splenocytes with Tat-coated nanoparticles was comparable to that from mice immunized with Tat and Lipid A, and 3.3-fold greater than that from mice immunized with Tat and Alum. These studies warrant further investigation of these nanoparticles to enhance both antibody and cellular-based immune responses. | lld:pubmed |
| pubmed-article:15193389 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15193389 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15193389 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15193389 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15193389 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15193389 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15193389 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15193389 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15193389 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15193389 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15193389 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15193389 | pubmed:issn | 0264-410X | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:CuiZhengrongZ | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:MumperRussell... | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:PatelJignaJ | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:NathAvindraA | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:WoodwardJerol... | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:TuzovaMarinaM | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:RayPhillipP | lld:pubmed |
| pubmed-article:15193389 | pubmed:author | pubmed-author:PhillipsRyanR | lld:pubmed |
| pubmed-article:15193389 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15193389 | pubmed:day | 30 | lld:pubmed |
| pubmed-article:15193389 | pubmed:volume | 22 | lld:pubmed |
| pubmed-article:15193389 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15193389 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15193389 | pubmed:pagination | 2631-40 | lld:pubmed |
| pubmed-article:15193389 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:meshHeading | pubmed-meshheading:15193389... | lld:pubmed |
| pubmed-article:15193389 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15193389 | pubmed:articleTitle | Strong T cell type-1 immune responses to HIV-1 Tat (1-72) protein-coated nanoparticles. | lld:pubmed |
| pubmed-article:15193389 | pubmed:affiliation | Center for Pharmaceutical Science and Technology, College of Pharmacy, University of Kentucky, Lexington, KY 40536-0082, USA. | lld:pubmed |
| pubmed-article:15193389 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15193389 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15193389 | lld:pubmed |
