| pubmed-article:15167797 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15167797 | lifeskim:mentions | umls-concept:C0009450 | lld:lifeskim |
| pubmed-article:15167797 | lifeskim:mentions | umls-concept:C0796344 | lld:lifeskim |
| pubmed-article:15167797 | lifeskim:mentions | umls-concept:C0683509 | lld:lifeskim |
| pubmed-article:15167797 | lifeskim:mentions | umls-concept:C0450254 | lld:lifeskim |
| pubmed-article:15167797 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:15167797 | pubmed:dateCreated | 2004-5-28 | lld:pubmed |
| pubmed-article:15167797 | pubmed:abstractText | An integrated portable genetic analysis microsystem including PCR amplification and capillary electrophoretic (CE) analysis coupled with a compact instrument for electrical control and laser-excited fluorescence detection has been developed. The microdevice contains microfabricated heaters, temperature sensors, and membrane valves to provide controlled sample positioning and immobilization in 200-nL PCR chambers. The instrument incorporates a solid-state laser and confocal fluorescence detection optics, electronics for sensing and powering the PCR reactor, and high-voltage power supplies for conducting CE separations. The fluorescein-labeled PCR products are amplified and electrophoretically analyzed in a gel-filled microchannel in <10 min. We demonstrate the utility of this instrument by performing pathogen detection and genotyping directly from whole Escherichia coli and Staphylococcus aureus cells. The E. coli detection assay consists of a triplex PCR amplification targeting genes that encode 16S ribosomal RNA, the fliC flagellar antigen, and the sltI shigatoxin. Serial dilution demonstrates a limit of detection of 2-3 bacterial cells. The S. aureus assay uses a femA marker to identify cells as S. aureus and a mecA marker to probe for methicillin resistance. This integrated portable genomic analysis microsystem demonstrates the feasibility of performing rapid high-quality detection of pathogens and their antimicrobial drug resistance. | lld:pubmed |
| pubmed-article:15167797 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15167797 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15167797 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15167797 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15167797 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15167797 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15167797 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15167797 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15167797 | pubmed:issn | 0003-2700 | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:SensabaughG... | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:SchererJ RJR | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:MathiesR ARA | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:RileyL WLW | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:RamchandaniMM | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:BlazejR GRG | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:LagallyE TET | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:TorielloN MNM | lld:pubmed |
| pubmed-article:15167797 | pubmed:author | pubmed-author:DiepB ABA | lld:pubmed |
| pubmed-article:15167797 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15167797 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:15167797 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:15167797 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15167797 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15167797 | pubmed:pagination | 3162-70 | lld:pubmed |
| pubmed-article:15167797 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:15167797 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15167797 | pubmed:articleTitle | Integrated portable genetic analysis microsystem for pathogen/infectious disease detection. | lld:pubmed |
| pubmed-article:15167797 | pubmed:affiliation | Chemistry Department, University of California, Berkeley, California 94720, USA. | lld:pubmed |
| pubmed-article:15167797 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15167797 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15167797 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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