| pubmed-article:15160300 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15160300 | lifeskim:mentions | umls-concept:C0445625 | lld:lifeskim |
| pubmed-article:15160300 | lifeskim:mentions | umls-concept:C0025663 | lld:lifeskim |
| pubmed-article:15160300 | lifeskim:mentions | umls-concept:C0029983 | lld:lifeskim |
| pubmed-article:15160300 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:15160300 | lifeskim:mentions | umls-concept:C1521991 | lld:lifeskim |
| pubmed-article:15160300 | lifeskim:mentions | umls-concept:C0220825 | lld:lifeskim |
| pubmed-article:15160300 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:15160300 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:15160300 | pubmed:dateCreated | 2004-5-25 | lld:pubmed |
| pubmed-article:15160300 | pubmed:abstractText | Detection of oxacillin resistance in coagulase-negative staphylococci (C-NS) by phenotypic methods is often difficult. The present study compared the National Committee for Clinical Laboratory Standards (NCCLS) revised guidelines of phenotypic methods with a mecA-based polymerase chain reaction (PCR) for C-NS. Ninety clinical C-NS isolates were tested for oxacillin resistance by disk diffusion (1-microg disk), minimum inhibitory concentration (MIC) breakpoint (0.5 microg/ml) after 24 h, and mecA-based PCR. The sensitivity and specificity of disk diffusion was 80% and 93%, and the sensitivity and specificity of the MIC breakpoint after 24 h was 84% and 91%, respectively, against PCR as gold standard. Eleven strains (7 mecA-positive and 4 mecA-negative) showed discordant results between MIC breakpoint after 24 h and PCR. Six of the 7 mecA-positive and all 4 mecA-negative discordant strains had inducible oxacillin resistance and beta-lactamase hyperproduction, respectively. The present study concludes that inducible oxacillin resistance and beta-lactamase hyperproduction are the major causes of discordant results between phenotypic methods and mecA-based PCR, and need special attention. | lld:pubmed |
| pubmed-article:15160300 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15160300 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15160300 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15160300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15160300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15160300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15160300 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15160300 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15160300 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15160300 | pubmed:issn | 1341-321X | lld:pubmed |
| pubmed-article:15160300 | pubmed:author | pubmed-author:GhoshalUjjala... | lld:pubmed |
| pubmed-article:15160300 | pubmed:author | pubmed-author:PrasadKashi... | lld:pubmed |
| pubmed-article:15160300 | pubmed:author | pubmed-author:AyyagariArcha... | lld:pubmed |
| pubmed-article:15160300 | pubmed:author | pubmed-author:SinghManishaM | lld:pubmed |
| pubmed-article:15160300 | pubmed:author | pubmed-author:TiwariDinesh... | lld:pubmed |
| pubmed-article:15160300 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15160300 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:15160300 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15160300 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15160300 | pubmed:pagination | 86-9 | lld:pubmed |
| pubmed-article:15160300 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:meshHeading | pubmed-meshheading:15160300... | lld:pubmed |
| pubmed-article:15160300 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15160300 | pubmed:articleTitle | A comparative evaluation of phenotypic and molecular methods for the detection of oxacillin resistance in coagulase-negative staphylococci. | lld:pubmed |
| pubmed-article:15160300 | pubmed:affiliation | Department of Microbiology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow 226 014, India. | lld:pubmed |
| pubmed-article:15160300 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15160300 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:15160300 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15160300 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15160300 | lld:pubmed |
