| pubmed-article:15154600 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15154600 | lifeskim:mentions | umls-concept:C0370231 | lld:lifeskim |
| pubmed-article:15154600 | lifeskim:mentions | umls-concept:C0032176 | lld:lifeskim |
| pubmed-article:15154600 | lifeskim:mentions | umls-concept:C0016263 | lld:lifeskim |
| pubmed-article:15154600 | lifeskim:mentions | umls-concept:C1522492 | lld:lifeskim |
| pubmed-article:15154600 | lifeskim:mentions | umls-concept:C1709793 | lld:lifeskim |
| pubmed-article:15154600 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:15154600 | pubmed:dateCreated | 2004-5-24 | lld:pubmed |
| pubmed-article:15154600 | pubmed:abstractText | Platelet aggregation and microaggregate formation were measured in samples of stirred whole blood by flow cytometry. Blood samples were stirred in a multi-sample agitator with ADP, fixed and labelled with a platelet-specific CD42a-FITC fluorescent antibody. The blood was then diluted and applied directly to a flow cytometer. Platelets were identified using a gating procedure based on their expression of CD42a and then quantified. Aggregation was monitored as a fall in the number of single platelets. Both reversible and irreversible aggregation responses to ADP were determined and these were found to correlate directly with aggregation responses determined using a well-established single platelet counting technique using the Ultra-Flo 100 Whole Blood Platelet Counter. We found from flow cytometry that ADP-induced aggregation was coupled with a transient formation of platelet microaggregates over the initial 60 s following ADP addition. Assessment of single platelet loss by flow cytometry was found to be a reliable way of monitoring aggregation responses and provided new information on rapid microaggregate formation in ADP-stimulated blood. | lld:pubmed |
| pubmed-article:15154600 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15154600 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154600 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15154600 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154600 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15154600 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15154600 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15154600 | pubmed:issn | 0953-7104 | lld:pubmed |
| pubmed-article:15154600 | pubmed:author | pubmed-author:HeptinstallSS | lld:pubmed |
| pubmed-article:15154600 | pubmed:author | pubmed-author:MayJ AJA | lld:pubmed |
| pubmed-article:15154600 | pubmed:author | pubmed-author:FoxS CSC | lld:pubmed |
| pubmed-article:15154600 | pubmed:author | pubmed-author:JansonSS | lld:pubmed |
| pubmed-article:15154600 | pubmed:author | pubmed-author:SasaoHH | lld:pubmed |
| pubmed-article:15154600 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15154600 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:15154600 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15154600 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15154600 | pubmed:pagination | 85-93 | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:meshHeading | pubmed-meshheading:15154600... | lld:pubmed |
| pubmed-article:15154600 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15154600 | pubmed:articleTitle | Quantitation of platelet aggregation and microaggregate formation in whole blood by flow cytometry. | lld:pubmed |
| pubmed-article:15154600 | pubmed:affiliation | Cardiovascular Medicine, University Hospital, Queen's Medical Centre, Nottingham NG7 2UH, UK. sue.fox@nottingham.ac.uk | lld:pubmed |
| pubmed-article:15154600 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15154600 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
