pubmed-article:1514964 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:1514964 | lifeskim:mentions | umls-concept:C1882726 | lld:lifeskim |
pubmed-article:1514964 | lifeskim:mentions | umls-concept:C0010405 | lld:lifeskim |
pubmed-article:1514964 | lifeskim:mentions | umls-concept:C0449851 | lld:lifeskim |
pubmed-article:1514964 | lifeskim:mentions | umls-concept:C1527362 | lld:lifeskim |
pubmed-article:1514964 | lifeskim:mentions | umls-concept:C0205352 | lld:lifeskim |
pubmed-article:1514964 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:1514964 | pubmed:dateCreated | 1992-10-2 | lld:pubmed |
pubmed-article:1514964 | pubmed:abstractText | Time-dependent changes in the viability of rat liver graft during cold preservation with Euro-Collins solution were evaluated with NADH fluorometry. Correlation between the fluorometric analysis, 1-week survival rate after liver transplantation, and mitochondrial ATP synthesis activity in the early phase after transplantation was studied. Fluorometric study: Rat livers were preserved at 0 degree-4 degrees C for 0-48 h in Euro-Collins solution and then reperfused for 15 min with oxygenated Krebs-Henseleit solution at 4 degrees C. The amplitude (R x A) between the oxidized and the reduced steady-state NADH fluorometric trace and the velocity (R x V) of the trace were determined to evaluate the mitochondrial respiratory chain. The R x A and R x V remained at levels higher than 90% of control after 6-h preservation, while the R x A of the 9-h preservation group and the R x V of the 12-h preservation group decreased significantly compared with those of the control and the 6-h preservation group. Survival study: a 100% survival rate after transplantation was achieved in the 6-h preservation group, whereas the rates were 18.8% and 0% in the 9- and 12-h preservation groups respectively. These survival rates correlated closely with the time-dependent decrease of the fluorometric parameters. Study of mitochondrial phosphorylative activity and energy charge 3 h after transplantation: With fresh grafts, the decrease in hepatic energy charge after transplantation was reduced to 0.79 from the control value of 0.86 by a 30% increase in mitochondrial ATP synthesis ability.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
pubmed-article:1514964 | pubmed:language | eng | lld:pubmed |
pubmed-article:1514964 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1514964 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:1514964 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:1514964 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:1514964 | pubmed:month | Jul | lld:pubmed |
pubmed-article:1514964 | pubmed:issn | 0934-0874 | lld:pubmed |
pubmed-article:1514964 | pubmed:author | pubmed-author:TanakaAA | lld:pubmed |
pubmed-article:1514964 | pubmed:author | pubmed-author:OzawaKK | lld:pubmed |
pubmed-article:1514964 | pubmed:author | pubmed-author:OkamuraRR | lld:pubmed |
pubmed-article:1514964 | pubmed:author | pubmed-author:UyamaSS | lld:pubmed |
pubmed-article:1514964 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:1514964 | pubmed:volume | 5 | lld:pubmed |
pubmed-article:1514964 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:1514964 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:1514964 | pubmed:pagination | 165-9 | lld:pubmed |
pubmed-article:1514964 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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pubmed-article:1514964 | pubmed:year | 1992 | lld:pubmed |
pubmed-article:1514964 | pubmed:articleTitle | Low-temperature fluorometric technique for evaluating the viability of rat liver grafts after simple cold storage. | lld:pubmed |
pubmed-article:1514964 | pubmed:affiliation | Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan. | lld:pubmed |
pubmed-article:1514964 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:1514964 | pubmed:publicationType | In Vitro | lld:pubmed |
http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1514964 | lld:pubmed |