| pubmed-article:15087226 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15087226 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15087226 | lifeskim:mentions | umls-concept:C0682638 | lld:lifeskim |
| pubmed-article:15087226 | lifeskim:mentions | umls-concept:C0546881 | lld:lifeskim |
| pubmed-article:15087226 | lifeskim:mentions | umls-concept:C0205246 | lld:lifeskim |
| pubmed-article:15087226 | lifeskim:mentions | umls-concept:C0920508 | lld:lifeskim |
| pubmed-article:15087226 | lifeskim:mentions | umls-concept:C0332324 | lld:lifeskim |
| pubmed-article:15087226 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:15087226 | pubmed:dateCreated | 2004-4-16 | lld:pubmed |
| pubmed-article:15087226 | pubmed:abstractText | In the interest of better understanding the role of human memory B cells in protection against disease, we developed an assay to quantitate antigen-specific memory B cells in human blood. This assay utilizes a 6-day polyclonal stimulation of PBMC followed by an antigen-specific ELISPOT for the detection of memory B cells that have differentiated into antibody secreting cells (ASC) in vitro. We have used this assay to demonstrate that the anthrax vaccine (AVA; BioThrax) elicits a substantial population of protective-antigen (PA) specific memory B cells, and these B cells satisfy the canonical surface phenotype of human memory B cells: CD19(+)CD20(+)Ig(+)CD27(+). These anti-PA antigen-specific memory B cells are IgG(+) and represent up to 2% of circulating IgG(+) B cells. Furthermore, these results confirm that vaccine-elicited memory B cells reside in the CD27(+) B cell population. This ELISPOT-based system has been designed in a generalized manner, such that the assay can be rapidly adapted to detect human antigen-specific memory B cells of any given specificity. This method should be useful for quantitatively assessing the potency of vaccines and the longevity of B cell immunological memory to various vaccines or infectious diseases. | lld:pubmed |
| pubmed-article:15087226 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15087226 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15087226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15087226 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15087226 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:15087226 | pubmed:issn | 0022-1759 | lld:pubmed |
| pubmed-article:15087226 | pubmed:author | pubmed-author:AhmedRafiR | lld:pubmed |
| pubmed-article:15087226 | pubmed:author | pubmed-author:CrottyShaneS | lld:pubmed |
| pubmed-article:15087226 | pubmed:author | pubmed-author:GlidewellJohn... | lld:pubmed |
| pubmed-article:15087226 | pubmed:author | pubmed-author:AubertRachael... | lld:pubmed |
| pubmed-article:15087226 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15087226 | pubmed:volume | 286 | lld:pubmed |
| pubmed-article:15087226 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15087226 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15087226 | pubmed:pagination | 111-22 | lld:pubmed |
| pubmed-article:15087226 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:meshHeading | pubmed-meshheading:15087226... | lld:pubmed |
| pubmed-article:15087226 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15087226 | pubmed:articleTitle | Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system. | lld:pubmed |
| pubmed-article:15087226 | pubmed:affiliation | Emory Vaccine Center and Department of Microbiology and Immunology, Emory University School of Medicine, 1510 Clifton Road, Rm G-211, Atlanta, GA 30322, USA. shane@liai.org | lld:pubmed |
| pubmed-article:15087226 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15087226 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15087226 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15087226 | lld:pubmed |
