| pubmed-article:15075199 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0600253 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0014597 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C1332713 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0032433 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C1415831 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C2239666 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:15075199 | lifeskim:mentions | umls-concept:C0333668 | lld:lifeskim |
| pubmed-article:15075199 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:15075199 | pubmed:dateCreated | 2004-4-12 | lld:pubmed |
| pubmed-article:15075199 | pubmed:abstractText | Apoptosis plays a crucial role in maintenance of intestinal epithelial integrity and is highly regulated by numerous factors, including cellular polyamines. We recently showed that polyamines regulate nuclear factor (NF)-kappaB activity in normal intestinal epithelial (IEC-6) cells and that polyamine depletion activates NF-kappaB and promotes resistance to apoptosis. The current study went further to determine whether the inhibitors of apoptosis (IAP) family of proteins, c-IAP2 and XIAP, are downstream targets of activated NF-kappaB and play a role in antiapoptotic activity of polyamine depletion in IEC-6 cells. Depletion of cellular polyamines by alpha-difluoromethylornithine not only activated NF-kappaB activity but also increased expression of c-IAP2 and XIAP. Specific inhibition of NF-kappaB by the recombinant adenoviral vector containing IkappaBalpha superrepressor (AdIkappaBSR) prevented the induction of c-IAP2 and XIAP in polyamine-deficient cells. Decreased levels of c-IAP2 and XIAP proteins by inactivation of NF-kappaB through AdIkappaBSR infection or treatment with the specific inhibitor Smac also overcame the resistance of polyamine-depleted cells to apoptosis induced by the combination of tumor necrosis factor (TNF)-alpha and cycloheximide (CHX). Although polyamine depletion did not alter levels of procaspase-3 protein, it inhibited formation of the active caspase-3. Decreased levels of c-IAP2 and XIAP by Smac prevented the inhibitory effect of polyamine depletion on the cleavage of procaspase-3 to the active caspase-3. These results indicate that polyamine depletion increases expression of c-IAP2 and XIAP by activating NF-kappaB in intestinal epithelial cells. Increased c-IAP2 and XIAP after polyamine depletion induce the resistance to TNF-alpha/CHX-induced apoptosis, at least partially, through inhibition of the caspase-3 activity. | lld:pubmed |
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| pubmed-article:15075199 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15075199 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15075199 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:15075199 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15075199 | pubmed:month | May | lld:pubmed |
| pubmed-article:15075199 | pubmed:issn | 0363-6143 | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:RinG DGD | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:VosWimW | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:RaoJaladanki... | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:WangJian-Ying... | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:ZouTongtongT | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:StrauchEric... | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:ZhangHuifang... | lld:pubmed |
| pubmed-article:15075199 | pubmed:author | pubmed-author:BassBarbara... | lld:pubmed |
| pubmed-article:15075199 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15075199 | pubmed:volume | 286 | lld:pubmed |
| pubmed-article:15075199 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15075199 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15075199 | pubmed:pagination | C1009-18 | lld:pubmed |
| pubmed-article:15075199 | pubmed:dateRevised | 2008-5-14 | lld:pubmed |
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| pubmed-article:15075199 | pubmed:meshHeading | pubmed-meshheading:15075199... | lld:pubmed |
| pubmed-article:15075199 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15075199 | pubmed:articleTitle | NF-kappaB-mediated IAP expression induces resistance of intestinal epithelial cells to apoptosis after polyamine depletion. | lld:pubmed |
| pubmed-article:15075199 | pubmed:affiliation | Dept. of Surgery, Baltimore Veterans Affairs Medical Center, 10 North Greene St., Baltimore, MD 21201, USA. jwang@smail.umaryland.edu | lld:pubmed |
| pubmed-article:15075199 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15075199 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15075199 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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