| pubmed-article:15063609 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15063609 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:15063609 | lifeskim:mentions | umls-concept:C0521009 | lld:lifeskim |
| pubmed-article:15063609 | lifeskim:mentions | umls-concept:C0041243 | lld:lifeskim |
| pubmed-article:15063609 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:15063609 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:15063609 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:15063609 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:15063609 | pubmed:dateCreated | 2004-4-5 | lld:pubmed |
| pubmed-article:15063609 | pubmed:abstractText | The expression of recombinant trypsinogens from different mammalian origins in Escherichia coli typically leads to the formation of insoluble aggregates. This work describes the high level expression of human trypsinogen 1 in E. coli using the T7 expression system. Direct expression of trypsinogen was not possible, but the N-terminal fusion of the first 11 amino acids of the T7 protein 10 resulted in an expression level of 200 mg g(-1) bacterial dry mass. A refolding procedure was optimized, and a method using continuous feed of denatured product was developed. Thus the working concentration of trypsinogen could be raised four-fold, while the yield of active protein could be maintained at 20-35%. The refolded trypsinogen was converted to trypsin by autocatalytic activation, and the utility for the detachment of mammalian cells in culture was proven. | lld:pubmed |
| pubmed-article:15063609 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15063609 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15063609 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15063609 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15063609 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15063609 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15063609 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15063609 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15063609 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15063609 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:15063609 | pubmed:issn | 0168-1656 | lld:pubmed |
| pubmed-article:15063609 | pubmed:author | pubmed-author:KatingerHerma... | lld:pubmed |
| pubmed-article:15063609 | pubmed:author | pubmed-author:Vorauer-UhlKa... | lld:pubmed |
| pubmed-article:15063609 | pubmed:author | pubmed-author:MattanovichDi... | lld:pubmed |
| pubmed-article:15063609 | pubmed:author | pubmed-author:HohenblumHube... | lld:pubmed |
| pubmed-article:15063609 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15063609 | pubmed:day | 8 | lld:pubmed |
| pubmed-article:15063609 | pubmed:volume | 109 | lld:pubmed |
| pubmed-article:15063609 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15063609 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15063609 | pubmed:pagination | 3-11 | lld:pubmed |
| pubmed-article:15063609 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:15063609 | pubmed:meshHeading | pubmed-meshheading:15063609... | lld:pubmed |
| pubmed-article:15063609 | pubmed:meshHeading | pubmed-meshheading:15063609... | lld:pubmed |
| pubmed-article:15063609 | pubmed:meshHeading | pubmed-meshheading:15063609... | lld:pubmed |
| pubmed-article:15063609 | pubmed:meshHeading | pubmed-meshheading:15063609... | lld:pubmed |
| pubmed-article:15063609 | pubmed:meshHeading | pubmed-meshheading:15063609... | lld:pubmed |
| pubmed-article:15063609 | pubmed:meshHeading | pubmed-meshheading:15063609... | lld:pubmed |
| pubmed-article:15063609 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15063609 | pubmed:articleTitle | Bacterial expression and refolding of human trypsinogen. | lld:pubmed |
| pubmed-article:15063609 | pubmed:affiliation | Institute of Applied Microbiology, University of Natural Resources and Applied Life Sciences, Vienna, Austria. | lld:pubmed |
| pubmed-article:15063609 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15063609 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15063609 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15063609 | lld:pubmed |
